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Basic Blue 148: A Rapid Stain for T Helper Cells

Biotechnic & Histochemistry , Volume 70 (2) – Jan 1, 1995

Details

Publisher
Informa UK Ltd
Copyright
© 1995 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted
Subject
Original Article
ISSN
1052-0295
eISSN
1473-7760
D.O.I.
10.3109/10520299509108325
Publisher site
See Article on Publisher Site

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Basic Blue 148: A Rapid Stain for T Helper Cells

Abstract

After brief exposure to an aqueous solution of the oxazine textile dye C. I. basic blue 148 following fixation in 37% formalin, 95% ethanol and glacial acetic acid, T helper cell nuclei and cytoplasm in specimens of peripheral blood displayed a deep red-violet color. No other cell in normal blood or bone marrow specimens showed intense staining of this type. The total staining time is 1 min. Basic blue 148 stain is a promising technique for hematology and immunology laboratories as a rapid screening test for T helper cells in blood specimens using a microscopic slide and ordinary incandescent illumination.
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