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A Modification to Facilitate Attachment of Large Object Tables to the Jung Tetrander I Microtome

A Modification to Facilitate Attachment of Large Object Tables to the Jung Tetrander I Microtome 312 STAIN TECHNOLOGY water drop on the slide. Each section is located in a definite area of the grid. The slide is dried for twelve hours at room temperature. After the water drops have evaporated, the sections adhere smoothly and evenly to the slide’s surface. Using our staining technique (0.1 % toluidine blue in Siirenson’s phosphate buffer pH 7.6 at room temperature for approximately one hour) the sections (Epon) do not become detached, although we observed that in a more alkaline staining solution they have a tendency to do so. After staining, preparation of the sections can be completed in the customary manner. Acknowledgement. This work was supported by Deutsche Forschungsgemeinschaft-SFB 89 - Kardiologie - GGttingen. REFERENCES Burnett, B. R. 1975. A new method for serially mounting resin sections (Spurr) for light microscopy. Stain Technol. 50: 288-290. Nadelhaft, 1. and Rose, L. M. 1974. Serial sectioning of Epon-embedded material for autoradiography. Stain Technol. 49: 178-181. Rohde, C. J . 1965. Serial sectioning from plastic-embedded specimens; arthropods in methacrylate. Stain Technol. 40: 43-44. A MODIFICATION TO FACILITATE ATTACHMENT LARGE OF OBJECT TABLES THE TO JUNG TETRANDER I MICROTOME L. WINSOR, School o j Biological Sciences, James Cook University of http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnic & Histochemistry Informa Healthcare

A Modification to Facilitate Attachment of Large Object Tables to the Jung Tetrander I Microtome

Abstract

312 STAIN TECHNOLOGY water drop on the slide. Each section is located in a definite area of the grid. The slide is dried for twelve hours at room temperature. After the water drops have evaporated, the sections adhere smoothly and evenly to the slide’s surface. Using our staining technique (0.1 % toluidine blue in Siirenson’s phosphate buffer pH 7.6 at room temperature for approximately one hour) the sections (Epon) do not become detached, although we observed that in a more alkaline staining solution they have a tendency to do so. After staining, preparation of the sections can be completed in the customary manner. Acknowledgement. This work was supported by Deutsche Forschungsgemeinschaft-SFB 89 - Kardiologie - GGttingen. REFERENCES Burnett, B. R. 1975. A new method for serially mounting resin sections (Spurr) for light microscopy. Stain Technol. 50: 288-290. Nadelhaft, 1. and Rose, L. M. 1974. Serial sectioning of Epon-embedded material for autoradiography. Stain Technol. 49: 178-181. Rohde, C. J . 1965. Serial sectioning from plastic-embedded specimens; arthropods in methacrylate. Stain Technol. 40: 43-44. A MODIFICATION TO FACILITATE ATTACHMENT LARGE OF OBJECT TABLES THE TO JUNG TETRANDER I MICROTOME L. WINSOR, School o j Biological Sciences, James Cook University of
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