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A Modification to Facilitate Attachment of Large Object Tables to the Jung Tetrander I Microtome

Biotechnic & Histochemistry , Volume 51 (6) – Jan 1, 1976

Details

Publisher
Informa UK Ltd
Copyright
© 1976 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted
Subject
Miscellaneous Article
ISSN
1052-0295
eISSN
1473-7760
D.O.I.
10.3109/10520297609116734
Publisher site
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A Modification to Facilitate Attachment of Large Object Tables to the Jung Tetrander I Microtome

Abstract

312 STAIN TECHNOLOGY water drop on the slide. Each section is located in a definite area of the grid. The slide is dried for twelve hours at room temperature. After the water drops have evaporated, the sections adhere smoothly and evenly to the slide’s surface. Using our staining technique (0.1 % toluidine blue in Siirenson’s phosphate buffer pH 7.6 at room temperature for approximately one hour) the sections (Epon) do not become detached, although we observed that in a more alkaline staining solution they have a tendency to do so. After staining, preparation of the sections can be completed in the customary manner. Acknowledgement. This work was supported by Deutsche Forschungsgemeinschaft-SFB 89 - Kardiologie - GGttingen. REFERENCES Burnett, B. R. 1975. A new method for serially mounting resin sections (Spurr) for light microscopy. Stain Technol. 50: 288-290. Nadelhaft, 1. and Rose, L. M. 1974. Serial sectioning of Epon-embedded material for autoradiography. Stain Technol. 49: 178-181. Rohde, C. J . 1965. Serial sectioning from plastic-embedded specimens; arthropods in methacrylate. Stain Technol. 40: 43-44. A MODIFICATION TO FACILITATE ATTACHMENT LARGE OF OBJECT TABLES THE TO JUNG TETRANDER I MICROTOME L. WINSOR, School o j Biological Sciences, James Cook University of
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