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Journals Genetics Volume 189 Issue 4

Mutations at the APM1 and APM2 loci in the green alga Chlamydomonas reinhardtii confer resistance to phosphorothioamidate and dinitroaniline herbicides. Genetic interactions between apm1 and apm2 mutations suggest an interaction between the gene products. We identified the APM1 and APM2 genes using a map-based cloning strategy. Genomic DNA fragments containing only the DNJ1 gene encoding a type I Hsp40 protein rescue apm1 mutant phenotypes, conferring sensitivity to the herbicides and rescuing a temperature-sensitive growth defect. Lesions at five apm1 alleles include missense mutations and nucleotide insertions and deletions that result in altered proteins or very low levels of gene expression. The HSP70A gene, encoding a cytosolic Hsp70 protein known to interact with Hsp40 proteins, maps near the APM2 locus. Missense mutations found in three apm2 alleles predict altered Hsp70 proteins. Genomic fragments containing the HSP70A gene rescue apm2 mutant phenotypes. The results suggest that a client of the Hsp70–Hsp40 chaperone complex may function to increase microtubule dynamics in Chlamydomonas cells. Failure of the chaperone system to recognize or fold the client protein(s) results in increased microtubule stability and resistance to the microtubule-destabilizing effect of the herbicides. The lack of redundancy of genes encoding cytosolic Hsp70 and Hsp40 type I proteins in Chlamydomonas makes it a uniquely valuable system for genetic analysis of the function of the Hsp70 chaperone complex. Received August 2, 2011. Accepted August 19, 2011. Copyright © 2011 by the Genetics Society of America « Previous | Next Article » Table of Contents This Article Published online before print September 21, 2011 , doi: 10.1534/genetics.111.133587 Genetics December 1, 2011 vol. 189 no. 4 1249-1260 » Abstract Full Text Full Text (PDF) Supporting Information Classifications Investigations Cellular Genetics Services Email this article to a friend Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Download to citation manager Citing Articles Load citing article information Google Scholar Articles by Silflow, C. D. PubMed PubMed citation Articles by Silflow, C. D. Related Content Load related web page information This Months's Issue January 2012, 190 (1) Alert me to new issues of Genetics ABOUT SUBSCRIBE INFO FOR AUTHORS ONLINE SUBMISSION EDITORIAL BOARD CONTACT ALERTS HELP ADVERTISING RSS Help Contact Us International Access Link Print ISSN: 0016-6731 Online ISSN: 1943-2631 Copyright © 2012 by the Genetics Society of America phone: 412-268-1812 fax: 412-268-1813 email: genetics-gsa@andrew.cmu.edu

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The Hsp70 and Hsp40 Chaperones Influence Microtubule Stability in Chlamydomonas

Silflow, Carolyn D.; Sun, Xiaoqing; Haas, Nancy A.; Foley, Joseph W.; Lefebvre, Paul A.
Genetics , Volume 189 (4): 1249
Genetics Society of America – Dec 1, 2011

More Info
  • Publisher Genetics Society of America
  • Copyright Copyright © 2011 by the Genetics Society of America
  • ISSN 0016-6731
  • eISSN 1943-2631
  • D.O.I. 10.1534/genetics.111.133587
  • Publisher site Get PDF  

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