Truncated isoform of mouse αT-catenin is testis-restricted in expression and function Steven Goossens * ,† , Barbara Janssens * ,1 , Griet Vanpoucke * ,2 , Riet De Rycke * ,† , Jolanda van Hengel * ,† and Frans van Roy * ,† ,3 * Department for Molecular Biomedical Research, VIB-Ghent University, Ghent, Belgium; and † Department of Molecular Biology, Ghent University, Ghent, Belgium 3 Correspondence: Department for Molecular Biomedical Research, VIB-Ghent University, Technologiepark 927, B-9052 Ghent, Belgium. E-mail: f.vanroy@dmbr.ugent.be αT-Catenin is a recently identified member of the α-catenin family of cell-cell adhesion molecules. For decades it was thought that α-catenins mediate solid cell-cell adhesion by linking the cadherin-mediated cell-cell adhesion complex with the actin cytoskeleton. However, the roles of α-catenins in this classical adhesion model have been questioned recently. αT-Catenin has a restricted expression pattern, in contrast to the ubiquitously expressed αE-catenin. High levels of αT-catenin were detected in heart and testis. Northern and Western blot experiments indicated that besides the standard full-length αT-catenin transcript, smaller alternative transcripts are expressed in testis. We report the cloning of two alternative transcripts of the mouse αT-catenin gene (transcript-B and -X), both of which are expressed in a testis-restricted manner from two putative alternative promoters. Alternative transcript-X encodes a smaller protein, isoform-X, which lacks the amino-terminal ß-catenin binding domain of the standard mouse αT-catenin protein, and is therefore unable to restore cell-cell adhesion in an α-catenin-negative colon carcinoma cell line. Immunohistochemical analysis showed specific localization of the αT-catenin isoform-X in the differentiating germ cells. In contrast to the standard full-length αT-catenin protein, this shortened isoform-X can bind to l-afadin, an important component of the nectin/afadin/ponsin adhesion complex that reportedly is essential for spermatogenesis.—Goossens, S., Janssens, B., Vanpoucke, G., De Rycke, R., van Hengel, J., van Roy, F. Truncated isoform of mouse αT-catenin is testis-restricted in expression and function. Key Words: afadin • testis • spermatogenesis
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Truncated isoform of mouse αT-catenin is testis-restricted in expression and function
Goossens, Steven; Janssens, Barbara; Vanpoucke, Griet; De Rycke, Riet; van Hengel, Jolanda; van Roy, Frans
Follow The FASEB Journal
, Volume 21 (3): 647
Fed of American Socs for Experimental Biology – Mar 1, 2007
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