Transgenic plant production of Cyanovirin-N, an HIV microbicide Amy Sexton 1 , Pascal M. Drake, Naheed Mahmood, Sarah J. Harman, Robin J. Shattock and Julian K-C. Ma Centre for Infection, Department of Cellular and Molecular Medicine, St. George’s University of London, London, UK 1 Correspondence: Centre for Infection, Department of Cellular and Molecular Medicine, St. George’s University of London, Cranmer Terrace, London SW17 0RE, UK. E-mail: a.sexton@sgul.ac.uk <h3>SPECIFIC AIMS</h3> To provide proof of concept for the use of a plant expression system to produce Cyanovirin-N (CV-N), a topical HIV microbicide candidate. This would be advantageous for microbicide production because it provides opportunities for economic scale up, as well as potentially overcoming existing problems of aggregation associated with Escherichia coli production of CV-N. <h3>PRINCIPAL FINDINGS</h3> <h3>1. Transgenic tobacco plants expressing recombinant CV-N were generated</h3> rCV-N was recovered from the highest expressing lines at levels approaching 1% of the tobacco plant’s total soluble protein <h3>2. The plant-derived CV-N is functional</h3> This was demonstrated by specific gp120 binding activity from a variety of HIV isolates and protection of T cells from in vitro HIV infection <h3>3. Plant-derived CV-N is expressed almost entirely in the preferred monomeric form</h3> 4. CV-N is rhizosecreted
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Transgenic plant production of Cyanovirin-N, an HIV microbicide
Sexton, Amy; Drake, Pascal M.; Mahmood, Naheed; Harman, Sarah J.; Shattock, Robin J.; Ma, Julian K-C.
Follow The FASEB Journal
, Volume 20 (2): 356
Fed of American Socs for Experimental Biology – Feb 1, 2006
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