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Journals The FASEB Journal Volume 20 Issue 3

The histone chaperone ASF1 localizes to active DNA replication forks to mediate efficient DNA replication Laura L. Schulz and Jessica K. Tyler 1 Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Aurora, Colorado, USA 1 Correspondence: Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center at Fitzsimons, Mail Stop 8101, PO Box 6511, Aurora, CO 80045, USA. E-mail: jessica.tyler@uchsc.edu <h3>SPECIFIC AIM</h3> The purpose of our study was to address the function of the histone chaperone Anti-silencing function 1 (ASF1) in metazoan cells by studying its localization and the consequences of RNA interference-mediated knockdown of Drosophila melanogaster ASF1 (dASF1). <h3>PRINCIPAL FINDINGS</h3> <h3>1. Loss of dASF1 causes slow cell proliferation</h3> To investigate the function of ASF1, RNA interference (RNAi) was used to reduce levels of dASF1 protein (knockdown) in Drosophila S2 cells in culture. The exposure of cells to double-stranded RNA (dsRNA) homologous to Drosophila ASF1 resulted in the knockdown of ASF1 protein to undetectable levels from day 2 to 8 after dsRNA exposure as measured by Western blot. Knockdown of dASF1 protein levels correlated with almost complete loss of nuclear dASF1 signal by immunofluorescence in all cells on day 2 after dsRNA

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The histone chaperone ASF1 localizes to active DNA replication forks to mediate efficient DNA replication

Schulz, Laura L.; Tyler, Jessica K.
The FASEB Journal , Volume 20 (3): 488
Fed of American Socs for Experimental Biology – Mar 1, 2006

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