The effect of inflammation on the generation of plasma DNA from dead and dying cells in the peritoneum Ning Jiang and David S. Pisetsky 1 Division of Rheumatology, Duke University Medical Center, and Medical Research Service, Durham VA Hospital, Durham, North Carolina 1 Correspondence: Durham VA Medical Center, Box 151G, 508 Fulton St., Durham, NC 27705. E-mail: dpiset@acpub.duke.edu To assess the effects of inflammation on the generation of circulating DNA from dead and dying cells, plasma DNA levels were determined in BALB/c mice, administered apoptotic or necrotic Jurkat cells following induction of peritonitis by treatment with thioglycollate (TG), peptone (PT), or sodium periodate (NaIO 4 ). In mice receiving TG or NaIO 4 , plasma DNA levels following intraperitoneal administration of Jurkat cells were significantly reduced compared with controls, whereas they were not affected in mice receiving PT. To determine the basis of these differences, the cellular composition of peritoneal fluids prior to the administration of the dead cells was analyzed. Among agents tested, TG administration led to the largest increase in cells, both neutrophils and monocytes. As shown by flow cytometry, the exudates contained apoptotic neutrophils and macrophages, with the highest levels in the TG-induced exudates. Analysis of DNA and caspase 3 in the fluids also showed differences. TG exudates showed increases in DNA and capase 3, while NaIO 4 -induced exudates had an increase only in DNA. Fluid from PT-treated mice did not have increases in DNA or caspase 3. Together, these results indicate that prior inflammation can affect the generation of blood DNA from apoptotic or necrotic cells, although this effect may vary depending on the composition of the exudates with respect to cells as well as DNA. Key Words: apoptosis • necrosis • neutrophils • peritonitis
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The effect of inflammation on the generation of plasma DNA from dead and dying cells in the peritoneum
Jiang, Ning; Pisetsky, David S.
Follow Journal of Leukocyte Biology
, Volume 77 (3): 296
Fed of American Socs for Experimental Biology – Mar 1, 2005
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