A novel CD11c lo CD11b hi MHC-II – CD8α – dendritic-like cell (L-DC) develops in cocultures of bone marrow over splenic stroma. L-DCs are distinct from other DC subsets and have potential importance in spleen for immunity to blood-borne antigens. As production is maintained in cultures for >12 months, L-DC development evidently depends on self-renewing progenitors. To improve this culture system, highly purified HSCs were sorted from bone marrow and used to establish cocultures. Nonadherent cells produced were analyzed for surface marker expression and capacity to activate/inhibit T cells. Cocultures produced a pure population of L-DCs for up to 12 months, which were strong activators of CD8 + T cells. The in vitro production of a pure population of L-DCs from HSCs—in numbers amenable to in vitro assays of function and development—therefore represents an important advance. hematopoiesis progenitors multiparameter FACS Footnotes B6 C57BL/6J cDC conventional DC F + /F – Flt3 + /Flt3 – subsets, respectively Flt3L fms-like tyrosine kinase-3 ligand FSC forward scatter HSC hematopoietic stem cell HSPC hematopoietic stem/progenitor cell KLS ckit + Lin – Sca1 + L-DC long-term culture DC Lin lineage OVA-FITC FITC-conjugated ovalbumin pDC plasmacytoid DC PI propidium iodide sDMEM supplemented DMEM medium SSC side scatter STX3 murine splenic stromal cell line Received June 20, 2011. Revision received October 5, 2011. Accepted October 20, 2011. © 2012 Society for Leukocyte Biology Facebook Google+ LinkedIn Mendeley Reddit StumbleUpon Technorati Twitter What's this? « Previous | Next Article » Table of Contents This Article Published online before print November 10, 2011 , doi: 10.1189/jlb.0611302 February 2012 Journal of Leukocyte Biology vol. 91 no. 2 341-346 » Abstract Full Text Full Text (PDF) All Versions of this Article: jlb.0611302v1 jlb.0611302v2 91/2/341 most recent Classifications Technical Advance Services Email this article to a colleague Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in PubMed Download to citation manager Citing Articles Load citing article information Google Scholar Articles by Hinton, R. A. Articles by O'Neill, H. C. PubMed PubMed citation Articles by Hinton, R. A. Articles by O'Neill, H. C. Related Content Load related web page information Sharing Email this article to a colleague Facebook Google+ LinkedIn Mendeley Reddit StumbleUpon Technorati Twitter What's this? Current Issue February 2012, 91 (2) From the Cover Editorials: Tim-3 puts on the brakes. Editorials: T cell memory, bone marrow, and aging: the good news. Spotlight on Leading Edge Research: Tim-3 regulates pro- and anti-inflammatory cytokine expression in human CD14+ monocytes. Spotlight on Leading Edge Research: The impact of aging on memory T cell phenotype and function in the human bone marrow. Reviews: Lipid-cytokine-chemokine cascades orchestrate leukocyte recruitment in inflammation. Reviews: Non-human primate dendritic cells. Alert me to new issues of Journal of Leukocyte Biology About JLB Submit Manuscripts Instructions for Authors Information for Reviewers Editorial Board Editorial Policies Subscriptions Librarian's Resource Advertising Press Room Copyright Permissions Feedback Join SLB SLB Annual Meeting Copyright © 2012 by the Society for Leukocyte Biology Print ISSN: 0741-5400 Online ISSN: 1938-3673 var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-23108860-1"); pageTracker._trackPageview();

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