Sphingosine 1-phosphate regulates myogenic differentiation: a major role for S1P 2 receptor Chiara Donati * ,† , Elisabetta Meacci * ,† , Francesca Nuti * , Laura Becciolini * , Marta Farnararo * and Paola Bruni * ,† ,1 * Dipartimento di Scienze Biochimiche and † Istituto Interuniversitario di Miologia (IIM), Università degli Studi di Firenze, Florence, Italy 1 Correspondence: Dipartimento di Scienze Biochimiche, Viale G.B. Morgagni 50, Florence 50134, Italy. E-mail: paola.bruni@unifi.it <h3>SPECIFIC AIMS</h3> The present study investigated whether the bioactive lipid sphingosine 1-phosphate (S1P) can influence myogenic differentiation, the receptor subtype(s) responsible for the biological response to the bioactive sphingolipid and the molecular mechanisms involved. <h3>PRINCIPAL FINDINGS</h3> <h3>1. S1P inhibits serum-induced proliferation and promotes cell cycle exit and myogenic differentiation of C2C12 cells</h3> The effect of S1P on myoblast proliferation was evaluated by labeled thymidine incorporation and MTS dye reduction assay in control and serum-stimulated myoblasts. S1P (1 µM) did not affect basal cell proliferation but reduced the DNA synthesis promoted by 10% fetal calf serum (FCS) evaluated by [ 3 H]thymidine incorporation (<h3>Fig. 1</h3> A) and MTS dye reduction assay experiments (Fig. 1 B ). The antiproliferative effect of S1P was confirmed by S1P-induced down-regulation
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Sphingosine 1-phosphate regulates myogenic differentiation: a major role for S1P2 receptor
Donati, Chiara; Meacci, Elisabetta; Nuti, Francesca; Becciolini, Laura; Farnararo, Marta; Bruni, Paola
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, Volume 19 (3): 449
Fed of American Socs for Experimental Biology – Mar 1, 2005
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