Roles and origins of leukocyte lipid bodies: proteomic and ultrastructural studies Hsiao-Ching Wan * ,1 , Rossana C. N. Melo * ,† ,1 , Zhoung Jin * , Ann M. Dvorak ‡ and Peter F. Weller * ,2 Departments of * Medicine and ‡ Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA; and † Department of Biology, Federal University of Juiz de Fora, UFJF, Juiz de Fora, MG, Brazil 2 Correspondence: Beth Israel Deaconess Medical Center, DA-617, 330 Brookline Ave., Boston, MA 02215, USA. E-mail: pweller@bidmc.harvard.edu Lipid bodies (LBs), multifunctional organelles present in most eukaryotic cells, are sites of eicosanoid formation in leukocytes; but little is known about the composition of leukocyte LBs or the biogenesis and internal structures of LBs from mammalian cells. Proteomic analyses of LBs purified from human monocytic U937 cells detected, common to LBs in other cells, proteins involved in cholesterol and triglyceride metabolism, Rab GTPases, and many membrane and endoplasmic reticulum (ER)-associated proteins. Newly lipid body (LB)-associated proteins included MRP-14, potentially involved in arachidonate transport, and ribosomal subunit proteins and translation regulatory proteins. Ultrastructurally, in U937 cells as well as human neutrophils and eosinophils, ribosomes are attached to and distributed within LBs, and LBs contain extensive ER-like membranes. The presence of ribosomes, ER-like membranes and many membrane-associated and ER luminal proteins within LBs, supports a new model by which enveloped ER-membranes and domains form LBs and indicates that LBs may be sites of protein synthesis.—Wan, H-C., Melo, R. C. N., Jin, Z., Dvorak, A. M., Weller, P. F. Roles and origins of leukocyte lipid bodies: proteomic and ultrastructural studies. Key Words: eosinophils • neutrophils • lipid bodies • lipid droplets • endoplasmic reticulum • U937 cells
End of preview. The entire article is 12 pages. To view the full-text, please rent this article to continue.
/lp/fed-of-american-socs-for-experimental-biology/roles-and-origins-of-leukocyte-lipid-bodies-proteomic-and-2GTA3HveRp
Welcome to DeepDyve! Rent Premier Research Articles and Save Up to 90%
Roles and origins of leukocyte lipid bodies: proteomic and ultrastructural studies
Wan, Hsiao-Ching; Melo, Rossana C. N.; Jin, Zhoung; Dvorak, Ann M.; Weller, Peter F.
Follow The FASEB Journal
, Volume 21 (1): 167
Fed of American Socs for Experimental Biology – Jan 1, 2007
More Info
More Like This Article
View All dataSource[]=actageo&dataSource[]=aspet&dataSource[]=aaos&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aea&dataSource[]=aip&dataSource[]=ajnr&dataSource[]=ams&dataSource[]=aps_physical&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=apha&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=aspb&dataSource[]=avs&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=berghahn&dataSource[]=cabi&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=du_press&dataSource[]=esa&dataSource[]=eu_press&dataSource[]=elsevier&dataSource[]=emerald&dataSource[]=ejtr&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=imanager&dataSource[]=imedpub&dataSource[]=informa_healthcare&dataSource[]=informs&dataSource[]=iop&dataSource[]=iucr&dataSource[]=iospress&dataSource[]=jbjs&dataSource[]=leftcoast&dataSource[]=lu_press&dataSource[]=mesharpe&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=nature&dataSource[]=oxford&dataSource[]=pier_professional&dataSource[]=pnas&dataSource[]=portlandpress&dataSource[]=psyc_articles&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=rsna&dataSource[]=rockefeller&dataSource[]=rcn&dataSource[]=ria&dataSource[]=rsc&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer_journal&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=wiley&dataSource[]=pct
© 2012 DeepDyve, Inc. All rights reserved.
Terms of Service | Privacy Policy
