Enter a sentence, or cut and paste a paragraph

Refine

Refine

Refine to these subject areas:

  • Select All | Select None

Advanced Filters »

Refine

  • Advanced Filters:

  • to

  • Specific Data Sources:

    All Edit

    Select All  |  Select None

Reset filters

Journals The FASEB Journal August 2009

Bookmark

RGK GTPase-dependent CaV2.1 Ca2+ channel inhibition is independent of CaV{beta}-subunit-induced current potentiation

To view this document, you will need to have Adobe Flash Player 10 or above installed.
Please click here to install.

RGK (Rad-Gem-Rem) GTPases have been described as potent negative regulators of the Ca 2+ influx via high-threshold voltage-activated Ca 2+ channels. Recent work, mostly performed on Ca V 1.2 Ca 2+ channels, has highlighted the crucial role played by the channel auxiliary Ca V β subunits and identified several GTPase and β-subunit protein domains involved in this regulation. We now extend these conclusions by producing the first complete characterization of the effects of Gem, Rem, and Rem2 on the neuronal Ca V 2.1 Ca 2+ channels expressed with Ca V β 1 or Ca V β 2 subunits. Current inhibition is limited to a decrease in amplitude with no modification in the voltage dependence or kinetics of the current. We demonstrate that this inhibition can occur for Ca V β constructs with impaired capacity to induce current potentiation, but that it is lost for Ca V β constructs deleted for their β-interaction domain. The RGK C-terminal last 80 amino acids are sufficient to allow potent current inhibition and in vivo β-subunit/Gem interaction. Interestingly, although Gem and Gem carboxy-terminus induce a completely different pattern of β-subunit cellular localization, they both potently inhibit Ca V 2.1 channels. These data therefore set the status of neuronal Ca V 2.1 Ca 2+ channel inhibition by RGK GTPases, emphasizing the role of short amino acid sequences of both proteins in β-subunit binding and channel inhibition and revealing a new mechanism for channel inhibition.—Leyris, J.-P., Gondeau, C., Charnet, A., Delattre, C., Rousset, M., Cens, T., Charnet, P. RGK GTPase-dependent Ca V 2.1 Ca 2+ channel inhibition is independent of Ca V β-subunit-induced current potentiation.

To view this document, you will need to have Adobe Flash Player 10 or above installed.
Please click here to install.

This is a preview. The total pages displayed will be limited.

Rent for $0.99 FREE

Login

It seems like you have an account, please login to rent this article

Forgot your password?

Don't have an account yet? Sign up now!

To view the full-text of this article, sign up for a free DeepDyve account below.

A free Basic account also comes with
3 free rentals to help get you started.

It seems like you have an account, please login to rent this article

Just 30 seconds to go! Please check your inbox for a confirmation email to activate your account, then start using your 3 FREE rentals.

Learn more Existing user? Login here

Article Details
Leyris, J.-P.; Gondeau, C.; Charnet, A.; Delattre, C.; Rousset, M.; Cens, T.; Charnet, P.
The FASEB Journal , Volume 23 (8): 2627
Fed of American Socs for Experimental Biology – Aug 1, 2009
More Info

More Like This Article

View All dataSource[]=aspet&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aip&dataSource[]=ajnr&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=aspb&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=elsevier&dataSource[]=emerald&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=imedpub&dataSource[]=iucr&dataSource[]=iospress&dataSource[]=jbjs&dataSource[]=mesharpe&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=oxford&dataSource[]=pnas&dataSource[]=psyc_articles&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=rsna&dataSource[]=rockefeller&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=pct

Browse: Subject Areas | Journals | Publishers

Bookmark an Article

To bookmark an article, please log in first, or sign up for a DeepDyve account if you don't already have one.

OK

Subscribe to Journal Email Alerts

To subscribe to email alerts, please log in first, or sign up for a DeepDyve account if you don't already have one.

OK