Response to: "Regarding detection of hydrogen peroxide in cigarette smoke" Elaine M. Khan and Tzipora Goldkorn 1 Signal Transduction Laboratory, Department of Internal Medicine, University of California, School of Medicine, Davis, California, USA 1 Correspondence: E-mail: ttgoldkorn@ucdavis.edu The authors of the paper, "Epidermal growth factor receptor exposed to cigarette smoke is aberrantly activated and undergoes perinuclear trafficking," (1) would like to respond to the comment by Dr. I. B. Chatterjee regarding our methodology for the detection of H 2 O 2 from cigarette smoke (CS). Dr. Chatterjee writes that CS contains strong reducing agents which may convert the red ferrithiocyanate complex into colorless ferrothiocyanate, thereby rendering this method inappropriate for detecting H 2 O 2 in CS. We believe Dr. Chatterjee’s concerns are not applicable for the following reasons: 1) We did not experience any bleaching of the ferrithiocyanate complex in our assay using cell culture medium exposed to whole CS. 2) Dr. Chatterjee states that CS solution added to a solution of pure H 2 O 2 results in no significant change in absorbance at 480 nm. There are no details describing the nature of the CS solution used, and upon reading one of Dr. Chatterjee’s publications
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Response to: "Regarding detection of hydrogen peroxide in cigarette smoke"
Khan, Elaine M.; Goldkorn, Tzipora
Follow The FASEB Journal
, Volume 22 (11): 3755
Fed of American Socs for Experimental Biology – Nov 1, 2008
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