Regulation of capacitative calcium entries by α1-syntrophin: association of TRPC1 with dystrophin complex and the PDZ domain of α1-syntrophin Aurélie Vandebrouck * , Jessica Sabourin * , Jérôme Rivet † , Haouria Balghi * , Stéphane Sebille * , Alain Kitzis * ,† , Guy Raymond * , Christian Cognard * , Nicolas Bourmeyster * ,† and Bruno Constantin * ,1 * Institut de Physiologie et Biologie Cellulaire, CNRS, UMR-6187, University of Poitiers, and † LGCM CHU de Poitiers, Poitiers, France 1 Correspondence: Institut de Physiologie et Biologie Cellulaires, CNRS, UMR-6187, University of Poitiers, 86022 Poitiers, France. E-mail: bruno.constantin@univ-poitiers.fr Calcium mishandling in Duchenne dystrophic muscle suggested that dystrophin, a membrane-associated cytoskeleton protein, might regulate calcium signaling cascade such as calcium influx pathway. It was previously shown that abnormal calcium entries involve uncontrolled stretch-activated currents and store-operated Ca 2+ currents supported by TRPC1 channels. Moreover, our recent work demonstrated that reintroduction of minidystrophin in dystrophic myotubes restores normal capacitative calcium entries (CCEs). However, until now, no molecular link between the dystrophin complex and calcium entry channels has been described. This study is the first to show by coimmunoprecipitation assays the molecular association of TRPC1 with dystrophin and α1-syntrophin in muscle cells. TRPC1 was also associated with α1-syntrophin in dystrophic muscle cells independently of dystrophin. Furthermore, glutathione S -transferase (GST) pull-down assays showed that TRPC1 binds to the α1-syntrophin PDZ domain. Transfected recombinant α1-syntrophin formed a complex with TRPC1 channels and restored normal CCEs in dystrophic muscle cells. We suggest that normal regulation of CCEs in skeletal muscle depends on the association between TRPC1 channels and α1-syntrophin that may anchor the store-operated channels to the dystrophin-associated protein complex (DAPC). The loss of this molecular association could participate in the calcium alterations observed in dystrophic muscle cells. This study provides a new model for the regulation of calcium influx by interaction with the scaffold of the DAPC in muscle cells. —Vandebrouck, A., Sabourin, J., Rivet, J., Balghi, H., Sebille, S., Kitzis, A., Raymond, G., Cognard, C., Bourmeyster, N., Constantin, B. Regulation of capacitative calcium entries by α1-syntrophin: association of TRPC1 with dystrophin complex and the PDZ domain of α1-syntrophin. Key Words: dystrophic muscle • store-operated calcium entries • TRP channels • scaffolding proteins
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Regulation of capacitative calcium entries by α1-syntrophin: association of TRPC1 with dystrophin complex and the PDZ domain of α1-syntrophin
Vandebrouck, Aurélie; Sabourin, Jessica; Rivet, Jérôme; Balghi, Haouria; Sebille, Stéphane; Kitzis, Alain; Raymond, Guy; Cognard, Christian; Bourmeyster, Nicolas; Constantin, Bruno
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, Volume 21 (2): 608
Fed of American Socs for Experimental Biology – Feb 1, 2007
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