Enter a sentence, or cut and paste a paragraph

Refine

Refine

Refine to these subject areas:

  • Select All | Select None

Advanced Filters »

Advanced Filters

Refine to these subject areas:

  • Select All | Select None

Advanced Filters «

  • Find information that...

  • to

  • Specific Data Sources:

    All Edit

    Select All  |  Select None

Reset filters

Bookmark

Recombinant guinea pig CCL5 (RANTES) differentially modulates cytokine production in alveolar and peritoneal macrophages

To view this document, you will need to have Adobe Flash Player installed.
Please click here to install.

The CC chemokine ligand 5 (CCL5; regulated on activation, normal T expressed and secreted) is known to recruit and activate leukocytes; however, its role in altering the responses of host cells to a subsequent encounter with a microbial pathogen has rarely been studied. Recombinant guinea pig (rgp)CCL5 was prepared, and its influence on peritoneal and alveolar macrophage activation was examined by measuring cytokine and chemokine mRNA expression in cells stimulated with rgpCCL5 alone or exposed to rgpCCL5 prior to lipopolysaccharide (LPS) stimulation. Levels of mRNA for guinea pig tumor necrosis factor (TNF- ), interleukin (IL)-1ß, CCL2 (monocyte chemoattractant protein-1), and CXC chemokine ligand 8 (IL-8) were analyzed by reverse transcription followed by real-time polymerase chain reaction analysis using SYBR Green. Bioactive TNF- protein concentration was measured using the L929 bioassay. Both macrophage populations displayed significant enhancement of all the genes and TNF- protein levels when stimulated with rgpCCL5, except for CCL2 in alveolar macrophages. When peritoneal or alveolar macrophages were pretreated with rgpCCL5 for 2 h and then exposed to low concentrations of LPS, diminished cytokine and chemokine mRNA levels were apparent at 6 h compared with LPS alone. At the protein level, there was a reduction in TNF- protein at 6 h in the CCL5-pretreated cells compared with LPS alone. These results further support a role for CCL5 in macrophage activation in addition to chemotactic properties and suggest a role in regulating the inflammatory response to LPS in the guinea pig by modulating the production of proinflammatory cytokines by macrophages.

This is a preview. The total pages displayed will be limited.

Rent for $0.99 FREE

Login

It seems like you have an account, please login to rent this article

Forgot your password?

Don't have an account yet? Sign up now!

To view the full-text of this article, sign up for a free DeepDyve account below.

A free Basic account also comes with
5 free rentals to help get you started.

It seems like you have an account, please login to rent this article

Learn more Existing user? Login here

Article Details
Skwor, Troy A.; Cho, Hyosun; Cassidy, Craig; Yoshimura, Teizo; McMurray, David N.
Journal of Leukocyte Biology , Volume 76 (6): 1229
Fed of American Socs for Experimental Biology – Dec 1, 2004
More Info
  • Publisher Soc Leukocyte Biology
  • Copyright Copyright © 2004 by the Society for Leukocyte Biology.
  • ISSN 0741-5400
  • D.O.I. 10.1189/jlb.0704414

More Like This Article

View All dataSource[]=aspet&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aip&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=iucr&dataSource[]=jbjs&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=oxford&dataSource[]=pnas&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=psyc_articles&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=pct

Browse: Journals | Publishers

Bookmark an Article

To bookmark an article, please log in first, or sign up for a DeepDyve account if you don't already have one.

OK

Preview-Only Articles

"Preview Only" refers to those articles which can only be previewed but not rented because DeepDyve does not currently have permission from the publisher. If you would like to download and purchase Preview-Only articles, or any of our Premium articles, you may click the "Download Article from Publisher" link. DeepDyve will take you directly to the article at the publisher's website to complete your transaction.

OK