The Krebs cycle plays a fundamental role in cardiac energy production and is often implicated in the energetic imbalance characteristic of heart disease. In this study, we measured Krebs cycle flux in real time in perfused rat hearts using hyperpolarized magnetic resonance spectroscopy (MRS). 2- 13 CPyruvate was hyperpolarized and infused into isolated perfused hearts in both healthy and postischemic metabolic states. We followed the enzymatic conversion of pyruvate to lactate, acetylcarnitine, citrate, and glutamate with 1 s temporal resolution. The appearance of 13 C-labeled glutamate was delayed compared with that of other metabolites, indicating that Krebs cycle flux can be measured directly. The production of 13 C-labeled citrate and glutamate was decreased postischemia, as opposed to lactate, which was significantly elevated. These results showed that the control and fluxes of the Krebs cycle in heart disease can be studied using hyperpolarized 2- 13 Cpyruvate.—Schroeder, M. A., Atherton, H. J., Ball, D. R., Cole, M. A., Heather, L. C., Griffin, J. L., Clarke, K., Radda, G. K. Tyler, D. J. Real-time assessment of Krebs cycle metabolism using hyperpolarized 13 C magnetic resonance spectroscopy.
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Real-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy
Schroeder, Marie A.; Atherton, Helen J.; Ball, Daniel R.; Cole, Mark A.; Heather, Lisa C.; Griffin, Julian L.; Clarke, Kieran; Radda, George K.; Tyler, Damian J.
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, Volume 23 (8): 2529
Fed of American Socs for Experimental Biology – Aug 1, 2009
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