Purinergic inhibition of the epithelial Na + transport via hydrolysis of PIP 2 K. Kunzelmann * ,1 , T. Bachhuber * , R. Regeer † , D. Markovich † , J. Sun † and R. Schreiber * * Institut für Physiologie, Universität Regensburg, Regensburg, Germany; and † Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia 1 Correspondence: Institut für Physiologie, Universität Regensburg, Universitätsstrasse 31, Regensburg, Germany. E-mail: uqkkunze@mailbox.uq.edu.au <h3>SPECIFIC AIMS</h3> We examined inhibition of amiloride sensitive Na + absorption (I sc-Amil ) in native trachea and mouse collecting duct (M1) cells by stimulation of purinergic receptors. We tried to uncover the molecular mechanism by which hydrolysis of phosphatidylinositol bisphosphate (PIP 2 ) during stimulation of P2Y 2 receptors inactivates the epithelial Na + channel ENaC, which is in charge of Na + absorption. <h3>PRINCIPAL FINDINGS</h3> 1. Stimulation of apically located purinergic receptors by ATP inhibits amiloride sensitive Na + absorption in airway epithelia and in mouse collecting duct cells The aminoglycoside neomycin, which binds with high affinity to PIP 2 , suppressed inhibition of Na + absorption by ATP. 2. Inhibition of the phosphatidylinositol 4 (PI 4 ) kinase by wortmannin (10 µmol/L) or blocking the
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Purinergic inhibition of the epithelial Na+ transport via hydrolysis of PIP2
Kunzelmann, K.; Bachhuber, T.; Regeer, R.; Markovich, D.; Sun, J.; Schreiber, R.
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, Volume 19 (1): 142
Fed of American Socs for Experimental Biology – Jan 1, 2005
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