Production of germline transgenic chickens expressing enhanced green fluorescent protein using a MoMLV-based retrovirus vector Bon Chul Koo * ,1 , Mo Sun Kwon * ,1 , Bok Ryul Choi † ,1 , Jin-Hoi Kim † ,2 , Seong-Keun Cho † , Sea Hwan Sohn ‡ , Eun Jung Cho ‡ , Hoon Taek Lee § , Wonkyung Chang || , Iksoo Jeon || , Jin-Ki Park || , Jae Bok Park ¶ and Teoan Kim * ,3 * Department of Physiology, ¶ Department of Pathology, Catholic University of Daegu School of Medicine, Daegu, Korea; † Division of Applied Life Science, GyeongSang National University, Jinju, Korea; ‡ Department of Animal Science and Biotechnology, Jinju National University, Jinju, Korea; § Animal Resources Research Center, Konkuk University, Seoul, Korea; and || National Livestock Research Institute, RDA, Suwon, Korea 3 Correspondence: Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, Korea. E-mail: takim@cu.ac.kr The Moloney murine leukemia virus (MoMLV) -based retrovirus vector system has been used most often in gene transfer work, but has been known to cause silencing of the imported gene in transgenic animals. In the present study, using a MoMLV-based retrovirus vector, we successfully generated a new transgenic chicken line expressing high levels of enhanced green fluorescent protein (eGFP). The level of eGFP expression was conserved after germline transmission and as much as 100 µg of eGFP could be detected per 1 mg of tissue protein. DNA sequencing showed that the transgene had been integrated at chromosome 26 of the G 1 and G 2 generation transgenic chickens. Owing to the stable integration of the transgene, it is now feasible to produce G 3 generation of homozygous eGFP transgenic chickens that will provide 100% transgenic eggs. These results will help establish a useful transgenic chicken model system for studies of embryonic development and for efficient production of transgenic chickens as bioreactors.—Koo, B. C., Kwon, M. S., Choi, B. R., Kim, J-H., Cho, S-K., Sohn, S. H., Cho, E. J., Lee, H. T., Chang, W., Jeon, I., Park, J-K., Park, J. B., Kim, T. Production of germline transgenic chickens expressing enhanced green fluorescent protein using a MoMLV-based retrovirus vector. Key Words: Moloney murine leukemia virus
End of preview. The entire article is 10 pages. To view the full-text, please rent this article to continue.
/lp/fed-of-american-socs-for-experimental-biology/production-of-germline-transgenic-chickens-expressing-enhanced-green-0V8s0ohpR8
Welcome to DeepDyve! Rent Premier Research Articles and Save Up to 90%
Production of germline transgenic chickens expressing enhanced green fluorescent protein using a MoMLV-based retrovirus vector
Koo, Bon Chul; Kwon, Mo Sun; Choi, Bok Ryul; Kim, Jin-Hoi; Cho, Seong-Keun; Sohn, Sea Hwan; Cho, Eun Jung; Lee, Hoon Taek; Chang, Wonkyung; Jeon, Iksoo; Park, Jin-Ki; Park, Jae Bok; Kim, Teoan
Follow The FASEB Journal
, Volume 20 (13): 2251
Fed of American Socs for Experimental Biology – Nov 1, 2006
More Info
More Like This Article
View All dataSource[]=actageo&dataSource[]=aspet&dataSource[]=aaos&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aea&dataSource[]=aip&dataSource[]=ajnr&dataSource[]=ams&dataSource[]=aps_physical&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=apha&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=aspb&dataSource[]=avs&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=berghahn&dataSource[]=cabi&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=du_press&dataSource[]=esa&dataSource[]=eu_press&dataSource[]=elsevier&dataSource[]=emerald&dataSource[]=ejtr&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=imanager&dataSource[]=imedpub&dataSource[]=informa_healthcare&dataSource[]=informs&dataSource[]=iop&dataSource[]=iucr&dataSource[]=iospress&dataSource[]=jbjs&dataSource[]=leftcoast&dataSource[]=lu_press&dataSource[]=mesharpe&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=nature&dataSource[]=oxford&dataSource[]=pier_professional&dataSource[]=pnas&dataSource[]=portlandpress&dataSource[]=psyc_articles&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=rsna&dataSource[]=rockefeller&dataSource[]=rcn&dataSource[]=ria&dataSource[]=rsc&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer_journal&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=wiley&dataSource[]=pct
© 2012 DeepDyve, Inc. All rights reserved.
Terms of Service | Privacy Policy
