Nitric oxide elicits functional MMP-13 protein-tyrosine nitration during wound repair Tania R. Lizarbe * , Concepción García-Rama * , Carlos Tarín * , Marta Saura † , Enrique Calvo * , Juan Antonio López * , Carlos López-Otín ‡ , Alicia R. Folgueras ‡ , Santiago Lamas § and Carlos Zaragoza * ,1 * Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain; † Departamento de Fisiología, Facultad de Medicina, Universidad de Alcalá, Madrid, Spain; ‡ Departamento de Bioquimica, Instituto Universitario de Oncologia, Universidad de Oviedo, Oviedo, Spain; and § Centro de Investigaciones Biológicas, Instituto "Reina Sofía" de Investigaciones Nefrológicas, Madrid, Spain 1 Correspondence: Fundación CNIC, Melchor Fernández Almagro 3, 28029 Madrid, Spain. E-mail: czaragoza@cnic.es Nitric oxide (NO) plays a critical role in wound healing, in part by promoting angiogenesis. However, the precise repair pathways affected by NO are not well defined. We now show that NO regulates matrix metalloproteinase-13 (MMP-13) release during wound repair. We find that normally MMP-13 is kept inside endothelial cells by an association with caveolin-1. However, nitration of MMP-13 on tyrosine residue Y338 causes it to dissociate from caveolin-1 and be released from endothelial cells. We next explored the functional significance of MMP-13 nitration in vivo . Skin injury increases nitration of MMP-13 in mice. Skin wounds in inducible nitric oxide synthase knockout mice release less MMP-13 and heal more slowly than skin wounds in wild-type mice. Conversely, skin wounds in caveolin-1 knockout mice have increased NO production, increased MMP-13 nitration, and accelerated wound healing. Collectively, our data reveal a new pathway through which NO modulates wound repair: nitration of MMP-13 promotes its release from endothelial cells, where it accelerates angiogenesis and wound healing.—Lizarbe, T. R., García-Ram, C., Tarín, C., Saura, M., Calvo, E., López, J. A., López-Otín, C., Folgueras, A. R., Lamas, S., Zaragoza, C. Nitric oxide elicits functional MMP-13 protein-tyrosine nitration during wound repair. Key Words: metalloproteinases • collagenase-3 • inducible nitric oxide synthase • caveolin-1 • wound healing
End of preview. The entire article is 9 pages. To view the full-text, please rent this article to continue.
/lp/fed-of-american-socs-for-experimental-biology/nitric-oxide-elicits-functional-mmp-13-protein-tyrosine-nitration-8cfIvXUiaG
Welcome to DeepDyve! Rent Premier Research Articles and Save Up to 90%
Nitric oxide elicits functional MMP-13 protein-tyrosine nitration during wound repair
Lizarbe, Tania R.; García-Rama, Concepción; Tarín, Carlos; Saura, Marta; Calvo, Enrique; López, Juan Antonio; López-Otín, Carlos; Folgueras, Alicia R.; Lamas, Santiago; Zaragoza, Carlos
Follow The FASEB Journal
, Volume 22 (9): 3207
Fed of American Socs for Experimental Biology – Sep 1, 2008
More Info
More Like This Article
View All dataSource[]=actageo&dataSource[]=aspet&dataSource[]=aaos&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aea&dataSource[]=aip&dataSource[]=ajnr&dataSource[]=ams&dataSource[]=aps_physical&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=apha&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=aspb&dataSource[]=avs&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=berghahn&dataSource[]=cabi&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=du_press&dataSource[]=esa&dataSource[]=eu_press&dataSource[]=elsevier&dataSource[]=emerald&dataSource[]=ejtr&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=imanager&dataSource[]=imedpub&dataSource[]=informa_healthcare&dataSource[]=informs&dataSource[]=iop&dataSource[]=iucr&dataSource[]=iospress&dataSource[]=jbjs&dataSource[]=leftcoast&dataSource[]=lu_press&dataSource[]=mesharpe&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=nature&dataSource[]=oxford&dataSource[]=pier_professional&dataSource[]=pnas&dataSource[]=portlandpress&dataSource[]=psyc_articles&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=rsna&dataSource[]=rockefeller&dataSource[]=rcn&dataSource[]=ria&dataSource[]=rsc&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer_journal&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=wiley&dataSource[]=pct
© 2012 DeepDyve, Inc. All rights reserved.
Terms of Service | Privacy Policy
