Neuropeptide Y is produced in visceral adipose tissue and promotes proliferation of adipocyte precursor cells via the Y1 receptor Kaiping Yang * ,† ,‡ ,1 , Haiyan Guan * ,† ,‡ , Edith Arany ‡ ,§ , David J. Hill ‡ ,§ and Xiang Cao * ,† ,‡ * Children’s Health Research Institute; and † Department of Obstetrics and Gynecology, ‡ Department of Physiology and Pharmacology, and § Department of Medicine, Lawson Health Research Institute; University of Western Ontario, London, Ontario, Canada 1 Correspondence: Children’s Health Research Institute, Rm. A5–132, Victoria Research Laboratories,Westminster Campus, 800 Commissioners Rd. East, London, Ontario, Canada N6A 4G5. E-mail: kyang@uwo.ca Neuropeptide Y (NPY) is synthesized in neural tissue of the central and peripheral nervous systems and has a number of important functions besides regulating appetite and energy homeostasis. Here we identify a novel site of NPY biosynthesis and a role for NPY in promoting proliferation of adipocyte precursor cells. We show that NPY mRNA is not only expressed in visceral adipose tissue (VAT) but that its levels are up-regulated 6-fold in our early-life programmed rat model of increased visceral adiposity. This is accompanied by a parallel rise in NPY protein, demonstrating that VAT is a novel peripheral site of NPY biosynthesis. Furthermore, NPY mRNA expression is also elevated >2-fold in VAT of obese Zucker rats. Importantly, NPY stimulates proliferation of primary rat preadipocytes as well as 3T3-L1 preadipocytes in vitro . This mitogenic effect appears to be mediated by the Y1 receptor and involves the activation of extracellular related kinase 1/2. In addition, insulin and glucocorticoid up-regulate VAT NPY expression in lean but not obese Zucker rats. Taken together, these results suggest that an enhanced local expression of NPY within VAT may be a common feature of and contribute to the molecular mechanisms underlying increased visceral adiposity.—Yang, K., Guan, H., Arany, E., Hill, D. J., Cao, X. Neuropeptide Y is produced in visceral adipose tissue and promotes proliferation of adipocyte precursor cells via the Y1 receptor. Key Words: obesity • Zucker rat • adipogenesis • 3T3-L1 cells
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Neuropeptide Y is produced in visceral adipose tissue and promotes proliferation of adipocyte precursor cells via the Y1 receptor
Yang, Kaiping; Guan, Haiyan; Arany, Edith; Hill, David J.; Cao, Xiang
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, Volume 22 (7): 2452
Fed of American Socs for Experimental Biology – Jul 1, 2008
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