NADH oxidase signaling induces cyclooxygenase-2 expression during lipopolysaccharide stimulation in cardiomyocytes Tianqing Peng, Xiangru Lu and Qingping Feng 1 Cardiology Research Laboratory, Lawson Health Research Institute, London Health Sciences Centre; and Departments of Medicine, Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada 1 Correspondence: Department of Medicine, London Health Sciences Centre, Victoria Research Tower, 6 th Floor, 800 Commissioners Road, London, Ontario N6A 4G5, Canada. E-mail: qfeng@uwo.ca <h3>SPECIFIC AIMS</h3> The aim of the present study was to investigate NADH oxidase signaling mechanisms leading to cyclooxygenas-2 (COX-2) expression in cardiomyocytes during lipopolysaccharide (LPS) stimulation. The roles of gp91 phox -containing NADH oxidase, mitogen-activated protein kinases (MAPK), and nuclear factor (NF) -κB in LPS-induced COX-2 expression were studied in cultured neonatal mouse cardiomyocytes. <h3>PRINCIPAL FINDINGS</h3> <h3>1. Basal COX-2 expression in cultured neonatal cardiomyocytes</h3> Under normal culture conditions, COX-2 mRNA was detected by RT-PCR in cardiomyocytes. PGE 2 , an end product of COX-2, was detectable the culture medium, which was almost completely inhibited by a selective COX-2 inhibitor NS398 (20 µM). These data suggest that cardiomyocytes express basal levels of COX-2 and produce PGE 2 . <h3>2. LPS-induced COX-2 expression does not require de novo protein synthesis</h3> In
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NADH oxidase signaling induces cyclooxygenase-2 expression during lipopolysaccharide stimulation in cardiomyocytes
Peng, Tianqing; Lu, Xiangru; Feng, Qingping
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, Volume 19 (2): 293
Fed of American Socs for Experimental Biology – Feb 1, 2005
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