Mesenchymal stem cells from the outer ear: a novel adult stem cell model system for the study of adipogenesis Jong-Seop Rim, Randall L. Mynatt and Barbara Gawronska-Kozak 1 Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA 1 Correspondence: Pennington Biomedical Research Center, 6400 Perkins Rd., Baton Rouge, LA 70808, USA. E-mail: kozakb@pbrc.edu <h3>SPECIFIC AIMS</h3> We have identified ear mesenchymal stem cells (EMSC) as a novel source of adult stem cells from the external ears of mice. The objectives of the present study were to 1) investigate the conditions for commitment of EMSC to the adipogenic lineage; 2) compare adipogenesis of EMSC to that of the stromal-vascular (S-V) cells present in fat depots of mice; and 3) generate and characterize clones of EMSC. <h3>PRINCIPAL FINDINGS</h3> <h3>1. Adipogenic potential of EMSC</h3> Cells were collected from external ears of 3 different strains of mice (Hsd: Athymic Nude-nu, FVB, and C57BL/6J). At confluence, EMSC at each of 5 passages were stimulated to differentiate into adipocytes by adding differentiation medium (DMEM/F12 plus 4% FBS supplemented with 1.7 µM insulin, 1 µM dexamethasone, and 0.5 mM isobutyl-methylxanthine). After 5 days in differentiation medium, EMSC show an abundance of oil red O-stained lipid droplets. Virtually
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Mesenchymal stem cells from the outer ear: a novel adult stem cell model system for the study of adipogenesis
Rim, Jong-Seop; Mynatt, Randall L.; Gawronska-Kozak, Barbara
Follow The FASEB Journal
, Volume 19 (9): 1205
Fed of American Socs for Experimental Biology – Jul 1, 2005
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