Matrix-specific activation of Src and Rho initiates capillary morphogenesis of endothelial cells YANQIU LIU and DONALD R. SENGER 1 Division of Cancer Biology and Angiogenesis, Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA 1 Correspondence: Department of Pathology, Research North, Beth Israel Deaconess Medical Center, 99 Brookline Ave., Boston, MA 02215, USA. E-mail: dsenger@caregroup.harvard.edu Interstitial collagen I stimulates microvascular endothelial cells to form solid cords that imitate precapillary structures found during angiogenesis. Time-lapse microscopy identified cell retraction and disruption of cell–cell contacts as early critical steps in collagen I-induced capillary morphogenesis. These early stages paralleled collagen I activation of Src kinase and GTPase Rho through ß1 integrins. The Src inhibitor PP2, dominant-negative Src, and Rho inhibitor exoenzyme C3 transferase each inhibited collagen I induction of actin stress fibers that mediate cell retraction and each inhibited capillary morphogenesis. Collagen I also disrupted VE-cadherin from intercellular junctions through a Src-dependent mechanism; both the Src inhibitor PP2 and dominant-negative Src preserved VE-cadherin localization to regions of cell–cell contact. An active Src mutant disrupted VE-cadherin and cell–cell contacts similarly to collagen I. In sharp contrast, laminin-1 did not induce capillary morphogenesis, and laminin-1 did not induce activation of Src or Rho. Rather, laminin-1 induced persistent activation of the GTPase Rac. Thus, these studies identify activation of Src and Rho as key mechanisms by which collagen I provokes capillary morphogenesis of microvascular endothelial cells, and they define marked differences between the functions of collagen I and laminin-1 in regulating endothelial cell morphogenesis.—Liu, Y., Senger, D. R. Matrix-specific activation of Src and Rho initiates capillary morphogenesis of endothelial cells. Key Words: angiogenesis • collagen • laminin • ß1 integrins • VE-cadherin
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Matrix-specific activation of Src and Rho initiates capillary morphogenesis of endothelial cells
LIU, YANQIU; SENGER, DONALD R.
Follow The FASEB Journal
, Volume 18 (3): 457
Fed of American Socs for Experimental Biology – Mar 1, 2004
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