M protein-mediated plasminogen binding is essential for the virulence of an invasive Streptococcus pyogenes isolate M. L. Sanderson-Smith * ,† , K. Dinkla † , J. N. Cole * , A. J. Cork * , P. G. Maamary * , J. D. McArthur * , G. S. Chhatwal † and M. J. Walker * ,1 * School of Biological Sciences, University of Wollongong, Wollongong, New South Wales, Australia; and † Department of Microbial Pathogenesis, Helmholtz Centre for Infection Research, Braunschweig, Germany 1 Correspondence: School of Biological Sciences, University of Wollongong, Wollongong, NSW, 2522, Australia. E-mail: mwalker@uow.edu.au The human protease plasmin plays a crucial role in the capacity of the group A streptococcus (GAS; Streptococus pyogenes ) to initiate invasive disease. The GAS strain NS88.2 was isolated from a case of bacteremia from the Northern Territory of Australia, a region with high rates of GAS invasive disease. Mutagenesis of the NS88.2 plasminogen binding M protein Prp was undertaken to examine the contribution of plasminogen binding and cell surface plasmin acquisition to virulence. The isogenic mutant NS88.2 prp was engineered whereby four amino acid residues critical for plasminogen binding were converted to alanine codons in the GAS genome sequence. The mutated residues were reverse complemented to the wild-type sequence to construct GAS strain NS88.2 prpRC . In comparison to NS88.2 and NS88.2 prpRC , the NS88.2 prp mutant exhibited significantly reduced ability to bind human plasminogen and accumulate cell surface plasmin activity during growth in human plasma. Utilizing a humanized plasminogen mouse model of invasive infection, we demonstrate that the capacity to bind plasminogen and accumulate surface plasmin activity plays an essential role in GAS virulence.—Sanderson-Smith, M. L., Dinkla, K., Cole, J. N., Cork, A. J., Maamary, P. G., McArthur, J. D., Chhatwal, G. S., Walker, M. J. M protein-mediated plasminogen binding is essential for the virulence of an invasive Streptococcus pyogenes isolate. Key Words: plasmin • group A streptococcus • innate immunity
End of preview. The entire article is 8 pages. To view the full-text, please rent this article to continue.
/lp/fed-of-american-socs-for-experimental-biology/m-protein-mediated-plasminogen-binding-is-essential-for-the-virulence-Ru4IlbGsXN
Welcome to DeepDyve! Rent Premier Research Articles and Save Up to 90%
M protein-mediated plasminogen binding is essential for the virulence of an invasive Streptococcus pyogenes isolate
Sanderson-Smith, M. L.; Dinkla, K.; Cole, J. N.; Cork, A. J.; Maamary, P. G.; McArthur, J. D.; Chhatwal, G. S.; Walker, M. J.
Follow The FASEB Journal
, Volume 22 (8): 2715
Fed of American Socs for Experimental Biology – Aug 1, 2008
More Info
More Like This Article
View All dataSource[]=actageo&dataSource[]=aspet&dataSource[]=aaos&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aea&dataSource[]=aip&dataSource[]=ajnr&dataSource[]=ams&dataSource[]=aps_physical&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=apha&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=aspb&dataSource[]=avs&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=berghahn&dataSource[]=cabi&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=du_press&dataSource[]=esa&dataSource[]=eu_press&dataSource[]=elsevier&dataSource[]=emerald&dataSource[]=ejtr&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=imanager&dataSource[]=imedpub&dataSource[]=informa_healthcare&dataSource[]=informs&dataSource[]=iop&dataSource[]=iucr&dataSource[]=iospress&dataSource[]=jbjs&dataSource[]=leftcoast&dataSource[]=lu_press&dataSource[]=mesharpe&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=nature&dataSource[]=oxford&dataSource[]=pier_professional&dataSource[]=pnas&dataSource[]=portlandpress&dataSource[]=psyc_articles&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=rsna&dataSource[]=rockefeller&dataSource[]=rcn&dataSource[]=ria&dataSource[]=rsc&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer_journal&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=wiley&dataSource[]=pct
© 2012 DeepDyve, Inc. All rights reserved.
Terms of Service | Privacy Policy
