Leukotriene B 4 and lipoxin A 4 are regulatory signals for neural stem cell proliferation and differentiation Koichiro Wada * ,1 ,2 , Makoto Arita † ,1 , Atsushi Nakajima § , Kazufumi Katayama * , Chiho Kudo * , Yoshinori Kamisaki * and Charles N. Serhan 2 ,† * Department of Pharmacology, Graduate School of Dentistry, Osaka University, Osaka, Japan; † Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts, USA; and § The Third Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama, Japan 2 Correspondence: Department of Pharmacology, Graduate School of Dentistry, Osaka University, 1–8 Yamadaoka, Suita, Osaka 565-0871, Japan, E-mail: kwada@dent.osaka-u.ac-jp ; or C.N.S., Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s Hospital and Harvard Medical School, 20 Shattuck St., Thorn 724, Boston, MA 02115, USA. E-mail: cnserhan@zeus.bwh.harvard.edu Leukotrienes (LTs) and lipoxins (LXs) are lipid mediators that play a key role in regulating acute inflammatory responses. Their roles in neural stem cell (NSC) functions are of interest. We showed here that LTB 4 and LXA 4 regulated proliferation and differentiation of murine NSCs that were isolated from embryo brains. Proliferation of NSCs was stimulated by LTB 4 (3 to 100 nM) and blocked by receptor antagonist (IC 50 =2.7 µM). In contrast, LXA 4 , and its aspirin-triggered-15-epi-LXA 4 stable analog attenuated growth of NSCs at as little as 1 nM. Both lipoxygenase (LOX) inhibitors and LTB 4 receptor antagonists caused apoptosis and cell death. Gene chip analysis revealed that growth-related gene expressions such as epidermal growth factor (EGF) receptor, cyclin E, p27, and caspase 8 were tightly regulated by LTB 4 ; LXA 4 gave the opposite gene expressions. In addition to proliferation, LTB 4 induced differentiation of NSCs into neurons as monitored by neurite outgrowth and MAP2 expression. These results indicate for the first time that LTB 4 and LXA 4 directly regulate proliferation and differentiation of NSCs, suggesting these new pathways may be useful in restoring stem cells.—Wada, K., Arita, M., Nakajima, A., Katayama, K., Kudo, C., Kamisaki, Y., Serhan, C. N. Leukotriene B 4 and lipoxin A 4 are regulatory signals for neural stem cell proliferation and differentiation. Key Words: inflammatory mediators • neural inflammation • BLT receptor • ALX receptors • neuron
End of preview. The entire article is 8 pages. To view the full-text, please rent this article to continue.
/lp/fed-of-american-socs-for-experimental-biology/leukotriene-b4-and-lipoxin-a4-are-regulatory-signals-for-neural-stem-EqhOj0xk4O
Welcome to DeepDyve! Rent Premier Research Articles and Save Up to 90%
Leukotriene B4 and lipoxin A4 are regulatory signals for neural stem cell proliferation and differentiation
Wada, Koichiro; Arita, Makoto; Nakajima, Atsushi; Katayama, Kazufumi; Kudo, Chiho; Kamisaki, Yoshinori; Serhan, Charles N.
Follow The FASEB Journal
, Volume 20 (11): 1785
Fed of American Socs for Experimental Biology – Sep 1, 2006
More Info
More Like This Article
View All dataSource[]=actageo&dataSource[]=aspet&dataSource[]=aaos&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aea&dataSource[]=aip&dataSource[]=ajnr&dataSource[]=ams&dataSource[]=aps_physical&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=apha&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=aspb&dataSource[]=avs&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=berghahn&dataSource[]=cabi&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=du_press&dataSource[]=esa&dataSource[]=eu_press&dataSource[]=elsevier&dataSource[]=emerald&dataSource[]=ejtr&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=imanager&dataSource[]=imedpub&dataSource[]=informa_healthcare&dataSource[]=informs&dataSource[]=iop&dataSource[]=iucr&dataSource[]=iospress&dataSource[]=jbjs&dataSource[]=leftcoast&dataSource[]=lu_press&dataSource[]=mesharpe&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=nature&dataSource[]=oxford&dataSource[]=pier_professional&dataSource[]=pnas&dataSource[]=portlandpress&dataSource[]=psyc_articles&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=rsna&dataSource[]=rockefeller&dataSource[]=rcn&dataSource[]=ria&dataSource[]=rsc&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer_journal&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=wiley&dataSource[]=pct
© 2012 DeepDyve, Inc. All rights reserved.
Terms of Service | Privacy Policy
