This study tested the hypothesis that Vγ3 TCR-bearing T cells are influenced by LCs. Vγ3 T cells and LCs are located in the epidermis of mice. Vγ3 T cells represent the main T cell population in the skin epithelium and play a crucial role in maintaining the skin integrity, whereas LCs are professional APCs. Although Vγ3 T cells and LCs form an interdigitating network in the epidermis, not much is known about their reciprocal influence and/or interdependence. We used two different LC-deficient mouse models, in which LCs are constitutively or inducibly depleted, to investigate the role of LCs in maturation, homeostasis, and function of Vγ3 T cells. We show that Vγ3 T cell numbers are unaltered by LC deficiency, and Vγ3 T cells isolated from LC-deficient mice are phenotypically and upon in vitro stimulation, functionally indistinguishable from Vγ3 T cells isolated from WT mice based on their cytotoxic potential and cytokine production. Additionally, in vivo skin-wounding experiments show no major difference in response of Vγ3 T cells to wounding in the absence or presence of LCs. These observations indicate that Vγ3 T cells develop and function independently of LCs. skin Langerin DTA mice Langerin DTR mice LC deficiency Footnotes CD62L CD62 ligand 51 Cr 51 Chromium DT diphtheria toxin DTA diphtheria toxin subunit A DTR diphtheria toxin receptor MFI mean fluorescence intensity Rae-1 retinoic acid early inducible-1 Tg transgenic Received October 25, 2010. Revision received March 22, 2011. Accepted March 23, 2011. © 2011 Society for Leukocyte Biology Facebook Google+ LinkedIn Mendeley Reddit StumbleUpon Technorati Twitter What's this? « Previous | Next Article » Table of Contents This Article Published online before print April 12, 2011 , doi: 10.1189/jlb.1010581 July 2011 Journal of Leukocyte Biology vol. 90 no. 1 61-68 » Abstract Full Text Full Text (PDF) All Versions of this Article: jlb.1010581v1 90/1/61 most recent Classifications Cell Development, Differtiation, and Trafficking Services Email this article to a colleague Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in PubMed Download to citation manager Citing Articles Load citing article information Google Scholar Articles by Taveirne, S. Articles by Leclercq, G. Search for related content PubMed PubMed citation Articles by Taveirne, S. Articles by Leclercq, G. Related Content Load related web page information Sharing Email this article to a colleague Facebook Google+ LinkedIn Mendeley Reddit StumbleUpon Technorati Twitter What's this? Current Issue October 2011, 90 (4) From the Cover Editorials: Granulopoiesis versus monopoiesis: a consequence of transcription factors dancing with the right partners. Editorials: Developmental regulation of P-glycoprotein and HIV therapy: spare the thymus but spoil the child? Spotlight on Leading Edge Research: AP-1 protein induction during monopoiesis favors C/EBP: AP-1 heterodimers over C/EBP homodimerization and stimulates FosB transcription. Spotlight on Leading Edge Research: Developmental regulation of P-glycoprotein activity within thymocytes results in increased anti-HIV protease inhibitor activity. Reviews: Role of alloreactive KIR2DS1+ NK cells in haploidentical hematopoietic stem cell transplantation. Reviews: Dendritic cells in bacteria handling in the gut. Reviews: Metabolism, LXR/LXR ligands, and tumor immune escape. Reviews: Plasmacytoid dendritic cells and cancer. Reviews: TLR7/9 versus TLR3/MDA5 signaling during virus infections and diabetes. Reviews: Maternal KIR and fetal HLA-C: a fine balance. Technical Advance: Soluble OX40 molecule mimics regulatory T cell modulatory activity on FcεRI-dependent mast cell degranulation. Alert me to new issues of Journal of Leukocyte Biology About JLB Submit Manuscripts Instructions for Authors Information for Reviewers Editorial Board Editorial Policies Subscriptions Librarian's Resource Advertising Press Room Copyright Permissions Feedback Join SLB SLB Annual Meeting Copyright © 2011 by the Society for Leukocyte Biology Print ISSN: 0741-5400 Online ISSN: 1938-3673 var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-23108860-1"); pageTracker._trackPageview();

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