GFP reporter mouse models of UPS proteolytic function Kristina Lindsten * , Victoria Menéndez-Benito † , Maria G. Masucci * and Nico P. Dantuma † *Microbiology and Tumor Biology Center, †Department of Cell and Molecular Biology, The Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden Kumarapeli and co-workers presented in the FASEB journal an in vivo reporter model for the ubiquitin/proteasome system (UPS) (1) . Similarly to the strategy that we used previously for the generation of our transgenic reporter model (2) , they generated mice ubiquitously expressing a modified green fluorescent protein (GFP) that is constitutively targeted for proteasomal degradation. While we welcome new mouse models for the UPS, we regret that the authors, in an attempt to make a direct comparison of the two models based on the literature, misrepresented some data from our earlier study. The authors argue that the proteasome inhibitor provoked in their UPS reporter mice accumulation of the reporter in several tissues that did not respond in earlier experiments with our reporter mice (1) . In addition, it is stated that this ‘unresponsiveness’ of tissues is a serious shortcoming of our reporter system. The authors correctly state that they used the same proteasome
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GFP reporter mouse models of UPS proteolytic function
Lindsten, Kristina; Menéndez-Benito, Victoria; Masucci, Maria G.; Dantuma, Nico P.; Kumarapeli, Asangi R. K.; Horak, Kathleen M.; Zheng, Hanqiao; Wang, Xuejun
Follow The FASEB Journal
, Volume 20 (7): 1027
Fed of American Socs for Experimental Biology – May 1, 2006
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