Functional human corneal endothelial cell sheets harvested from temperature-responsive culture surfaces Taizo Sumide * , Kohji Nishida * ,1 , Masayuki Yamato † , Takeshi Ide * , Yasutaka Hayashida * , Katsuhiko Watanabe * , Joseph Yang † , Chinatsu Kohno † , Akihiko Kikuchi † , Naoyuki Maeda * , Hitoshi Watanabe * , Teruo Okano † and Yasuo Tano * * Department of Ophthalmology, Osaka University Medical School, Suita, Japan; and † Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan 1 Correspondence: Department of Ophthalmology, Osaka University Medical School, Room E7, 2-2 Yamadaoka, Suita 565-0871, Japan. E-mail: knishida@ophthal.med.osaka-u.ac.jp <h3>SPECIFIC AIM</h3> Our aim was to fabricate self-supporting, bioengineered human corneal endothelial cell (HCEC) sheets in vitro that retain native morphological and functional characteristics, and thus are suitable for corneal regenerative therapies. <h3>PRINCIPAL FINDINGS</h3> <h3>1. DNA synthesis of HCECs on temperature-responsive culture dishes</h3> For noninvasive cell sheet harvest that preserves cell viability, cell-to-cell junctions, extracellular matrix (ECM), and cell sheet contiguity, we applied unique temperature-responsive culture surfaces onto which an ultrathin layer of temperature-responsive polymer, poly ( N -isopropylacrylamide) (PIPAAm), is covalently grafted. Since PIPAAm is known to undergo a phase transition
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Functional human corneal endothelial cell sheets harvested from temperature-responsive culture surfaces
Sumide, Taizo; Nishida, Kohji; Yamato, Masayuki; Ide, Takeshi; Hayashida, Yasutaka; Watanabe, Katsuhiko; Yang, Joseph; Kohno, Chinatsu; Kikuchi, Akihiko; Maeda, Naoyuki; Watanabe, Hitoshi; Okano, Teruo; Tano, Yasuo
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, Volume 20 (2): 392
Fed of American Socs for Experimental Biology – Feb 1, 2006
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