Engineering the morphology and electrophysiological parameters of cultured neurons by microfluidic surface patterning ELENA V. ROMANOVA, KARI A. FOSSER, STANISLAV S. RUBAKHIN, RALPH G. NUZZO and JONATHAN V. SWEEDLER 1 Department of Chemistry, Beckman Institute and Frederick Seitz Materials Research Laboratory, University of Illinois, Urbana, Illinois, USA 1 Correspondence: Department of Chemistry, University of Illinois, 71 Roger Adams Lab, 600 S. Mathews Ave., Urbana, IL 61801, USA. E-mail: sweedler@scs.uiuc.edu <h3>SPECIFIC AIMS</h3> We wanted to know how localized extracellular stimuli control the shape and physiological properties of neurons. To answer these questions, we examined the morphological, biochemical and electrophysiological parameters of individual Aplysia neurons cultured in the chemically and spatially defined environment of micropatterned surfaces. <h3>PRINCIPAL FINDINGS</h3> <h3>1. The spatial features presented by a micropatterned substrate of growth permissive molecules determine the nerve cell morphology</h3> We find that patterns of covalently immobilized adhesion molecules, polylysine (PLL) and collagen IV, alternated with nonadhesive regions of BSA guide the neurite extension in cultured Aplysia neurons and modulate neuron structural characteristics. PLL and collagen IV have profoundly different effects on cell morphology in cultures on conventional uniform substrates. When presented as surface patterns, however, these growth permissive molecules induce changes in cellular
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Engineering the morphology and electrophysiological parameters of cultured neurons by microfluidic surface patterning
ROMANOVA, ELENA V.; FOSSER, KARI A.; RUBAKHIN, STANISLAV S.; NUZZO, RALPH G.; SWEEDLER, JONATHAN V.
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, Volume 18 (11): 1267
Fed of American Socs for Experimental Biology – Aug 1, 2004
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