Distinguishing fibroblast promigratory and procontractile growth factor environments in 3-D collagen matrices Hongmei Jiang, Sangmyung Rhee, Chin-Han Ho and Frederick Grinnell 1 Department of Cell Biology, University of Texas Southwestern Medical School, Dallas, Texas 1 Correspondence: Department of Cell Biology, University of Texas Southwestern Medical School, 5323 Harry Hines Boulevard, Dallas, TX 75390-9039, USA. E-mail: frederick.grinnell@utsouthwestern.edu Understanding growth factor function during wound repair is necessary for the development of therapeutic interventions to improve healing outcomes. In the current study, we compare the effects of serum and purified growth factors on human fibroblast function in three different collagen matrix models: cell migration in nested matrices, floating matrix contraction, and stressed-released matrix contraction. The results of these studies indicate that platelet-derived growth factor (PDGF) is unique in its capacity to promote cell migration. Serum, lysophosphatidic acid, sphingosine-1-phophate (S1P), and endothelin-1 promote stressed-released matrix contraction but not cell migration. In addition, we found that S1P inhibits fibroblast migration and treatment of serum to remove lipid growth factors or treatment of cells to interfere with S1P 2 receptor function increases serum promigratory activity. Our findings suggest that different sets of growth factors generate promigratory and procontractile tissue environments for fibroblasts and that the balance between PDGF and S1P is a key determinant of fibroblast promigratory activity.—Jiang, H., Rhee, S., Ho, C.-H., Grinnell, F. Distinguishing fibroblast promigratory and procontractile growth factor environments in 3-D collagen matrices. Key Words: cell migration • cell contraction • platelet-derived growth factor • lysophosphatidic acid • sphingosine-1-phosphate • wound repair
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Distinguishing fibroblast promigratory and procontractile growth factor environments in 3-D collagen matrices
Jiang, Hongmei; Rhee, Sangmyung; Ho, Chin-Han; Grinnell, Frederick
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, Volume 22 (7): 2151
Fed of American Socs for Experimental Biology – Jul 1, 2008
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