Direct inhibition of the mitochondrial permeability transition pore: a possible mechanism responsible for anti-apoptotic effects of melatonin 1 SHAIDA A. ANDRABI * , IQBAL SAYEED † , DETLEF SIEMEN † , GERALD WOLF * and THOMAS F. W. HORN * ,2 * Institute for Medical Neurobiology and † Department of Neurology Otto-von-Guericke University, Magdeburg, Germany 2 Correspondence: Leipziger St. 44, Haus 36, Magdeburg, D-39120 Germany. E-mail: thomas.horn@medizin.uni-magdeburg.de <h3>SPECIFIC AIM</h3> We investigate whether the pineal hormone melatonin directly inhibits potentially proapoptotic mitochondrial permeability transition pore (mtPTP) activity at single-channel and cellular levels. We examined whether such mtPTP inhibition by melatonin leads to decreased NMDA receptor-induced increase in cytosolic calcium ([Ca 2+ ] c ). <h3>PRINCIPAL FINDINGS</h3> 1. Melatonin reduces sustained [Ca 2+ ] c increase in primary neuronal cultures exposed to NMDA Upon stimulation of striatal neurons with 200 µM NMDA, we observed a fast increase in the fluo-4 fluorescence from a baseline intensity of 103.4 ± 4.3 (average of single cell arbitrary intensity values ± SE, n =5 cultures) to 282.3 ± 22.5 immediately after exposure to NMDA (<h3>Fig. 1</h3> ). The increased fluorescence, indicating an increase in [Ca 2+ ] c , was still high (284.3±16.2) 18
End of preview. The entire article is 3 pages. To view the full-text, please rent this article to continue.
/lp/fed-of-american-socs-for-experimental-biology/direct-inhibition-of-the-mitochondrial-permeability-transition-pore-a-YhSIWaltua
Welcome to DeepDyve! Rent Premier Research Articles and Save Up to 90%
Direct inhibition of the mitochondrial permeability transition pore: a possible mechanism responsible for anti-apoptotic effects of melatonin
ANDRABI, SHAIDA A.; SAYEED, IQBAL; SIEMEN, DETLEF; WOLF, GERALD; HORN, THOMAS F. W.
Follow The FASEB Journal
, Volume 18 (7): 869
Fed of American Socs for Experimental Biology – May 1, 2004
More Info
More Like This Article
View All dataSource[]=actageo&dataSource[]=aspet&dataSource[]=aaos&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aea&dataSource[]=aip&dataSource[]=ajnr&dataSource[]=ams&dataSource[]=aps_physical&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=apha&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=aspb&dataSource[]=avs&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=berghahn&dataSource[]=cabi&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=du_press&dataSource[]=esa&dataSource[]=eu_press&dataSource[]=elsevier&dataSource[]=emerald&dataSource[]=ejtr&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=imanager&dataSource[]=imedpub&dataSource[]=informa_healthcare&dataSource[]=informs&dataSource[]=iop&dataSource[]=iucr&dataSource[]=iospress&dataSource[]=jbjs&dataSource[]=leftcoast&dataSource[]=lu_press&dataSource[]=mesharpe&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=nature&dataSource[]=oxford&dataSource[]=pier_professional&dataSource[]=pnas&dataSource[]=portlandpress&dataSource[]=psyc_articles&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=rsna&dataSource[]=rockefeller&dataSource[]=rcn&dataSource[]=ria&dataSource[]=rsc&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer_journal&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=wiley&dataSource[]=pct
© 2012 DeepDyve, Inc. All rights reserved.
Terms of Service | Privacy Policy
