Differential insertion of GPI-anchored GFPs into lipid rafts of live cells Daniel F. Legler * ,† ,1 , Marie-Agnès Doucey † , Pascal Schneider † , Laurence Chapatte † , Florent C. Bender † and Claude Bron † * Department of Biology, Division of Immunology, University of Konstanz, Konstanz, Germany; and † Institute of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Epalinges, Switzerland 1 Correspondence: Department of Biology, Division of Immunology, University of Konstanz, Universitätsstrasse 10, Room P1105, Konstanz 78457, Germany. E-mail: daniel.legler@uni-konstanz.de <h3>SPECIFIC AIM</h3> To design raft-specific fluorescent markers, we engineered different glycosylphosphatidyl-inositol (GPI) -anchored green fluorescent proteins (GFP-GPI) and studied their membrane localization upon cell transfection and after painting of live cells, which allows insertion of purified GFP-GPI into the plasma membrane. <h3>PRINCIPAL FINDINGS</h3> <h3>1. Distinct localization of different GFP-GPIs in lipid rafts</h3> Partitioning of proteins in cholesterol and sphingolipid-enriched plasma membrane microdomains, called lipid rafts, is critical for many signal transduction and protein sorting events. Certain proteins preferentially localize into lipid rafts—in particular, a group of functionally diverse cell surface proteins anchored in the outer leaflet of microdomains by a complex glycosylphosphatidyl-inositol (GPI) moiety. We used this property to generate an extracellular fluorescent marker
End of preview. The entire article is 3 pages. To view the full-text, please rent this article to continue.
/lp/fed-of-american-socs-for-experimental-biology/differential-insertion-of-gpi-anchored-gfps-into-lipid-rafts-of-live-UoPwXT1AUA
Welcome to DeepDyve! Rent Premier Research Articles and Save Up to 90%
Differential insertion of GPI-anchored GFPs into lipid rafts of live cells
Legler, Daniel F.; Doucey, Marie-Agnès; Schneider, Pascal; Chapatte, Laurence; Bender, Florent C.; Bron, Claude
Follow The FASEB Journal
, Volume 19 (1): 73
Fed of American Socs for Experimental Biology – Jan 1, 2005
More Info
More Like This Article
View All dataSource[]=actageo&dataSource[]=aspet&dataSource[]=aaos&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aea&dataSource[]=aip&dataSource[]=ajnr&dataSource[]=ams&dataSource[]=aps_physical&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=apha&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=aspb&dataSource[]=avs&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=berghahn&dataSource[]=cabi&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=du_press&dataSource[]=esa&dataSource[]=eu_press&dataSource[]=elsevier&dataSource[]=emerald&dataSource[]=ejtr&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=imanager&dataSource[]=imedpub&dataSource[]=informa_healthcare&dataSource[]=informs&dataSource[]=iop&dataSource[]=iucr&dataSource[]=iospress&dataSource[]=jbjs&dataSource[]=leftcoast&dataSource[]=lu_press&dataSource[]=mesharpe&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=nature&dataSource[]=oxford&dataSource[]=pier_professional&dataSource[]=pnas&dataSource[]=portlandpress&dataSource[]=psyc_articles&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=rsna&dataSource[]=rockefeller&dataSource[]=rcn&dataSource[]=ria&dataSource[]=rsc&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer_journal&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=wiley&dataSource[]=pct
© 2012 DeepDyve, Inc. All rights reserved.
Terms of Service | Privacy Policy
