Enter a sentence, or cut and paste a paragraph

Refine

Refine

Refine to these subject areas:

  • Select All | Select None

Advanced Filters »

Advanced Filters

Refine to these subject areas:

  • Select All | Select None

Advanced Filters «

  • Find information that...

  • to

  • Specific Data Sources:

    All Edit

    Select All  |  Select None

Reset filters

Bookmark

Dendritic cells differentiated in the presence of IFN-{beta} and IL-3 are potent inducers of an antigen-specific CD8+ T cell response

To view this document, you will need to have Adobe Flash Player installed.
Please click here to install.

Dendritic cells (DC) are professional antigen-presenting cells that are used in vaccine approaches to cancer. Classically, mature monocyte-derived DC are generated in vitro in the presence of interleukin (IL)-4, granulocyte macrophage-colony stimulating factor, and inflammatory cytokines (G4-DC). Recently, it has been described that DC can also be generated in the presence of IL-3 and interferon (IFN)-ß and that these DC are efficiently matured using polyriboinosinic polyribocytidylic acid (I3-DC). In this study, a series of in vitro experiments was performed to compare side-by-side I3-DC and G4-DC as vaccine adjuvants. Phenotypic characterization of the DC revealed differences in the expression of the monocyte marker CD14 and the maturation marker CD83. Low expression of CD14 and high expression of CD83 characterized G4-DC, whereas I3-DC displayed intermediate expression of CD14 and CD83. Both types of DC were as potent in the induction of allogeneic T cell proliferation. Upon CD40 ligation, G4-DC produced lower amounts of IFN- and pulmonary and activation-regulated chemokine, similar amounts of IL-6, macrophage-inflammatory protein (MIP)-1 , and MIP-1ß, and higher amounts of IL-12 p70, tumor necrosis factor , and MIP-3ß than I3-DC. We further evaluated whether the DC could be frozen/thawed without loss of cell number, viability, phenotype, and function. After freezing/thawing, 56.0% ± 9.0% of I3-DC and 77.0% ± 3.0% of G4-DC (n=9) were recovered as viable cells, displaying the same phenotype as their fresh counterparts. Finally, in vitro stimulations showed that fresh and frozen peptide-loaded I3-DC are more potent inducers of Melan-A-specific CD8 + T cell responses than G4-DC. The antigen-specific T cells were functional as shown in cytotoxicity and IFN- secretion assay.

This is a preview. The total pages displayed will be limited.

Rent for $0.99 FREE

Login

It seems like you have an account, please login to rent this article

Forgot your password?

Don't have an account yet? Sign up now!

To view the full-text of this article, sign up for a free DeepDyve account below.

A free Basic account also comes with
5 free rentals to help get you started.

It seems like you have an account, please login to rent this article

Learn more Existing user? Login here

Article Details
Breckpot, Karine; Corthals, Jurgen; Bonehill, Aude; Michiels, Annelies; Tuyaerts, Sandra; Aerts, Cindy; Heirman, Carlo; Thielemans, Kris
Journal of Leukocyte Biology , Volume 78 (4): 898
Fed of American Socs for Experimental Biology – Oct 1, 2005
More Info
  • Publisher Soc Leukocyte Biology
  • Copyright Copyright © 2005 by the Society for Leukocyte Biology.
  • ISSN 0741-5400
  • D.O.I. 10.1189/jlb.0105052

More Like This Article

View All dataSource[]=aspet&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aip&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=iucr&dataSource[]=jbjs&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=oxford&dataSource[]=pnas&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=psyc_articles&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=pct

Browse: Journals | Publishers

Bookmark an Article

To bookmark an article, please log in first, or sign up for a DeepDyve account if you don't already have one.

OK

Preview-Only Articles

"Preview Only" refers to those articles which can only be previewed but not rented because DeepDyve does not currently have permission from the publisher. If you would like to download and purchase Preview-Only articles, or any of our Premium articles, you may click the "Download Article from Publisher" link. DeepDyve will take you directly to the article at the publisher's website to complete your transaction.

OK