Definition of the critical domains required for homophilic targeting of mouse sidekick molecules Kayo Hayashi * ,† ,1 , Lewis Kaufman * , Michael D. Ross * and Paul E. Klotman * * Division of Nephrology, Mt. Sinai School of Medicine, New York, New York, USA; and † Division of Nephrology, Department of Internal Medicine, Juntendo University School of Medicine, Tokyo, Japan 1 Correspondence: Division of Nephrology, Mount Sinai School of Medicine, Annenberg Bldg., Room 23-38 One Gustave L. Levy Pl., New York, NY 10029, USA. E-mail: kayo.hayashi@mssm.edu <h3>SPECIFIC AIMS</h3> Homophilic binding of mouse sidekick-1 and sidekick-2 proteins, orthologous transmembrane proteins of the immunoglobulin superfamily, has been shown to be important in targeting and stabilizing the developing neuronal synapse and in the pathogenesis of HIV-associated nephropathy. The aims of the current work were to: 1) characterize the homophilic binding properties of sidekick molecules in cells; and 2) define the critical domains responsible for targeting and stabilizing these homophilic interactions. <h3>PRINCIPAL FINDINGS</h3> 1. <h3>Sidekicks mediate homophilic adhesion</h3> To determine whether homophilic binding by sidekicks mediates cell adhesion, we cloned murine sidekicks 1 and 2. Sidekick proteins were then overexpressed in HEK 293T cells and cell aggregation assays were performed.
End of preview. The entire article is 3 pages. To view the full-text, please rent this article to continue.
/lp/fed-of-american-socs-for-experimental-biology/definition-of-the-critical-domains-required-for-homophilic-targeting-1Lp9fKPx2Y
Welcome to DeepDyve! Rent Premier Research Articles and Save Up to 90%
Definition of the critical domains required for homophilic targeting of mouse sidekick molecules
Hayashi, Kayo; Kaufman, Lewis; Ross, Michael D.; Klotman, Paul E.
Follow The FASEB Journal
, Volume 19 (6): 614
Fed of American Socs for Experimental Biology – Apr 1, 2005
More Info
More Like This Article
View All dataSource[]=actageo&dataSource[]=aspet&dataSource[]=aaos&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aea&dataSource[]=aip&dataSource[]=ajnr&dataSource[]=ams&dataSource[]=aps_physical&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=apha&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=aspb&dataSource[]=avs&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=berghahn&dataSource[]=cabi&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=du_press&dataSource[]=esa&dataSource[]=eu_press&dataSource[]=elsevier&dataSource[]=emerald&dataSource[]=ejtr&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=imanager&dataSource[]=imedpub&dataSource[]=informa_healthcare&dataSource[]=informs&dataSource[]=iop&dataSource[]=iucr&dataSource[]=iospress&dataSource[]=jbjs&dataSource[]=leftcoast&dataSource[]=lu_press&dataSource[]=mesharpe&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=nature&dataSource[]=oxford&dataSource[]=pier_professional&dataSource[]=pnas&dataSource[]=portlandpress&dataSource[]=psyc_articles&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=rsna&dataSource[]=rockefeller&dataSource[]=rcn&dataSource[]=ria&dataSource[]=rsc&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer_journal&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=wiley&dataSource[]=pct
© 2012 DeepDyve, Inc. All rights reserved.
Terms of Service | Privacy Policy
