Comprehensive measurement of chromosomal instability in cancer cells: combination of fluorescence in situ hybridization and cytokinesis-block micronucleus assay Jordi Camps * ,1 , Immaculada Ponsa * , Maria Ribas † , Esther Prat * , Josep Egozcue * , Miguel A. Peinado † and Rosa Miró * * Departament de Biologia Cel·lular, Fisiologia i Immunologia and Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, Spain; and † IDIBELL-Institut de Recerca Oncològica, Hospital Duran i Reynals, L’Hospitalet, Barcelona, Spain 1 Correspondence: Laboratori de Citogenètica, Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. E-mail: jordi.camps@uab.es <h3>SPECIFIC AIMS</h3> In the present study, we quantify the ongoing structural chromosome alterations in two archetypes of colorectal cancer cell lines: HCT116, and SW480 and its single subclones using the multicolor-FISH. The application of the cytokinesis-block micronucleus (CBMN) assay allowed a detailed measurement of numerical instability, to elucidate the origin of the aneuploidy and to predict the structural chromosome instability level by measuring the abnormal nuclear shape events by performing centromeric and pancentromeric FISH. <h3>PRINCIPAL FINDINGS</h3> <h3>1. Rates of structural chromosome instability</h3> Several efforts have been assessed to characterize and quantify the ongoing appearance of structural chromosome
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Comprehensive measurement of chromosomal instability in cancer cells: combination of fluorescence in situ hybridization and cytokinesis-block micronucleus assay
Camps, Jordi; Ponsa, Immaculada; Ribas, Maria; Prat, Esther; Egozcue, Josep; Peinado, Miguel A.; Miró, Rosa
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, Volume 19 (7): 828
Fed of American Socs for Experimental Biology – May 1, 2005
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