C1-TEN is a negative regulator of the Akt/PKB signal transduction pathway and inhibits cell survival, proliferation, and migration Sassan Hafizi 1 , Filiz Ibraimi and Björn Dahlbäck Department of Clinical Chemistry, Lund University, Wallenberg Laboratory, University Hospital Malmö, Malmö, Sweden 1 Correspondence: Department of Clinical Chemistry, Lund University, Wallenberg Laboratory, University Hospital Malmö, SE-205 02 Malmö, Sweden. E-mail: sassan.hafizi@med.mas.lu.se <h3>SPECIFIC AIMS</h3> We have investigated the effects of overexpression of a novel intracellular molecule, C1 domain-containing phosphatase and TENsin homologue (C1-TEN), on the phenotypic and intracellular signal transduction properties of mammalian HEK293 cells. <h3>PRINCIPAL FINDINGS</h3> <h3>1. Stable overexpression of C1-TEN caused a profound alteration in cellular phenotype</h3> Mock transfected cells (M-293) showed an identical morphology to untransfected cells, whereas C1-TEN-transfected cells (C-293) possessed rounder and smoother cell bodies and slightly longer processes. C-293 cells displayed weaker fluorescent staining of filamentous actin. Therefore, C1-TEN overexpression clearly alters cytoskeletal architecture in HEK293 cells. <h3>2. Reduced proliferation and migration of C1-TEN-overexpressing cells</h3> Stable expression of C1-TEN markedly suppressed the growth rate of 293 cells as compared with mock transfection in response to serum over 5 days. Cells overexpressing the active site cysteine mutant C231S showed a fully restored rate of proliferation. C-293
End of preview. The entire article is 3 pages. To view the full-text, please rent this article to continue.
/lp/fed-of-american-socs-for-experimental-biology/c1-ten-is-a-negative-regulator-of-the-akt-pkb-signal-transduction-Nwds0Akznq
Welcome to DeepDyve! Rent Premier Research Articles and Save Up to 90%
C1-TEN is a negative regulator of the Akt/PKB signal transduction pathway and inhibits cell survival, proliferation, and migration
Hafizi, Sassan; Ibraimi, Filiz; Dahlbäck, Björn
Follow The FASEB Journal
, Volume 19 (8): 971
Fed of American Socs for Experimental Biology – Jun 1, 2005
More Info
More Like This Article
View All dataSource[]=actageo&dataSource[]=aspet&dataSource[]=aaos&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aea&dataSource[]=aip&dataSource[]=ajnr&dataSource[]=ams&dataSource[]=aps_physical&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=apha&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=aspb&dataSource[]=avs&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=berghahn&dataSource[]=cabi&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=du_press&dataSource[]=esa&dataSource[]=eu_press&dataSource[]=elsevier&dataSource[]=emerald&dataSource[]=ejtr&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=imanager&dataSource[]=imedpub&dataSource[]=informa_healthcare&dataSource[]=informs&dataSource[]=iop&dataSource[]=iucr&dataSource[]=iospress&dataSource[]=jbjs&dataSource[]=leftcoast&dataSource[]=lu_press&dataSource[]=mesharpe&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=nature&dataSource[]=oxford&dataSource[]=pier_professional&dataSource[]=pnas&dataSource[]=portlandpress&dataSource[]=psyc_articles&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=rsna&dataSource[]=rockefeller&dataSource[]=rcn&dataSource[]=ria&dataSource[]=rsc&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer_journal&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=wiley&dataSource[]=pct
© 2012 DeepDyve, Inc. All rights reserved.
Terms of Service | Privacy Policy
