Enter a sentence, or cut and paste a paragraph

Refine

Refine

Refine to these subject areas:

  • Select All | Select None

Advanced Filters »

Refine

  • Advanced Filters:

  • to

  • Specific Data Sources:

    All Edit

    Select All  |  Select None

Reset filters

Journals The FASEB Journal October 2009

Bookmark

Ae2a,b-Deficient mice exhibit osteopetrosis of long bones but not of calvaria

To view this document, you will need to have Adobe Flash Player 10 or above installed.
Please click here to install.

Extracellular acidification by osteoclasts is essential to bone resorption. During proton pumping, intracellular pH (pH i ) is thought to be kept at a near-neutral level by chloride/bicarbonate exchange. Here we show that the Na + -independent chloride/bicarbonate anion exchanger 2 (Ae2) is relevant for this process in the osteoclasts from the long bones of Ae2 a,b –/– mice (deficient in the main isoforms Ae2a, Ae2b 1 , and Ae2b 2 ). Although the long bones of these mice had normal numbers of multinucleated osteoclasts, these cells lacked a ruffled border and displayed impaired bone resorption activity, resulting in an osteopetrotic phenotype of long bones. Moreover, in vitro osteoclastogenesis assays using long-bone marrow cells from Ae2 a,b –/– mice suggested a role for Ae2 in osteoclast formation, as fusion of preosteoclasts for the generation of active multinucleated osteoclasts was found to be slightly delayed. In contrast to the abnormalities observed in the long bones, the skull of Ae2 a,b –/– mice showed no alterations, indicating that calvaria osteoclasts may display normal resorptive activity. Microfluorimetric analysis of osteoclasts from normal mice showed that, in addition to Ae2 activity, calvaria osteoclasts—but not long-bone osteoclasts—possess a sodium-dependent bicarbonate transporting activity. Possibly, this might compensate for the absence of Ae2 in calvaria osteoclasts of Ae2 a,b –/– mice.—Jansen, I. D. C., Mardones, P., Lecanda, F., de Vries, T. J., Recalde, S., Hoeben, K. A., Schoenmaker, T., Ravesloot, J.-H., van Borren, M. M. G. J., van Eijden, T. M., Bronckers, A. L. J. J., Kellokumpu, S., Medina, J. F., Everts, V., Oude Elferink, R. P. J. Ae2 a,b -deficient mice exhibit osteopetrosis of long bones but not of calvaria.

To view this document, you will need to have Adobe Flash Player 10 or above installed.
Please click here to install.

This is a preview. The total pages displayed will be limited.

Rent for $0.99 FREE

Login

It seems like you have an account, please login to rent this article

Forgot your password?

Don't have an account yet? Sign up now!

To view the full-text of this article, sign up for a free DeepDyve account below.

A free Basic account also comes with
3 free rentals to help get you started.

It seems like you have an account, please login to rent this article

Just 30 seconds to go! Please check your inbox for a confirmation email to activate your account, then start using your 3 FREE rentals.

Learn more Existing user? Login here

Article Details
More Info

More Like This Article

View All dataSource[]=aspet&dataSource[]=aacc&dataSource[]=aacr&dataSource[]=aip&dataSource[]=ajnr&dataSource[]=appi_book&dataSource[]=appi_journal&dataSource[]=asip&dataSource[]=asm&dataSource[]=asn&dataSource[]=aspb&dataSource[]=annual_reviews&dataSource[]=arxiv&dataSource[]=acm&dataSource[]=clinical_trials&dataSource[]=dailymed&dataSource[]=degruyter&dataSource[]=elsevier&dataSource[]=emerald&dataSource[]=emea&dataSource[]=epo&dataSource[]=faseb&dataSource[]=gsa&dataSource[]=health_affairs&dataSource[]=hindawi&dataSource[]=imedpub&dataSource[]=iucr&dataSource[]=iospress&dataSource[]=jbjs&dataSource[]=mesharpe&dataSource[]=mary_ann_liebert&dataSource[]=medline&dataSource[]=mit_press&dataSource[]=oxford&dataSource[]=pnas&dataSource[]=psyc_articles&dataSource[]=psyc_books&dataSource[]=psyc_critiques&dataSource[]=plos_journal&dataSource[]=pubmed_central&dataSource[]=rsna&dataSource[]=rockefeller&dataSource[]=sage&dataSource[]=spie&dataSource[]=springer&dataSource[]=taylor_francis&dataSource[]=aps&dataSource[]=the_scientist&dataSource[]=uc_press&dataSource[]=uspto_abstract&dataSource[]=pct

Browse: Subject Areas | Journals | Publishers

Bookmark an Article

To bookmark an article, please log in first, or sign up for a DeepDyve account if you don't already have one.

OK

Subscribe to Journal Email Alerts

To subscribe to email alerts, please log in first, or sign up for a DeepDyve account if you don't already have one.

OK