A transport mechanism for NAADP in a rat basophilic cell line R. A. Billington 1 , E. A. Bellomo, E. M. Floriddia, J. Erriquez, C. Distasi and A. A. Genazzani DiSCAFF and DFB Center, Università del Piemonte Orientale, Novara, Italy 1 Correspondence: DiSCAFF, Via Bovio 6, Novara 28100, Italy. E-mail: billington@pharm.unipmn.it <h3>SPECIFIC AIMS</h3> The goals of this work were to investigate whether the second messenger NAADP could be translocated from the extracellular medium across the plasma membrane into the cytosol and have the potential to act as a paracrine/autocrine signal. <h3>PRINCIPAL FINDINGS</h3> <h3>1. RBL-2H3 cells possess a concentrative uptake mechanism for NAADP</h3> Incubation of RBL-2H3 cells with [ 32 P]NAADP resulted in a substantial accumulation of the radioactive second messenger in cells (6.6±0.6 fmol in 1 h when 125 fmol of [ 32 P]NAADP were added extracellularly) (Table 1 ). This accumulation was due to a transporter since 1) it was significantly abrogated at 4°C; 2) it was unaffected by pre- or postincubation with an excess nonradioactive NAADP; 3) nonradioactive NAADP competed for the uptake mechanism dose-dependently, while NAAD competed only modestly at 10 µM and NAD did not compete; 4) the mechanism was time and concentration dependent
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A transport mechanism for NAADP in a rat basophilic cell line
Billington, R. A.; Bellomo, E. A.; Floriddia, E. M.; Erriquez, J.; Distasi, C.; Genazzani, A. A.
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, Volume 20 (3): 521
Fed of American Socs for Experimental Biology – Mar 1, 2006
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