A simple assay for frequency of chromosome breaks and loss (micronuclei) by flow cytometry of human reticulocytes Tal Offer * , 1 , Emily Ho * ,† , 1 , Maret G. Traber † , Richard S. Bruno † , Frans A. Kuypers * and Bruce N. Ames * ,2 * Nutritional Genomics Center, Children’s Hospital Oakland Research Institute, Oakland, California, USA; and † Department of Nutrition & Food Management and Linus Pauling Institute, Oregon State University, Corvallis, Oregon, USA 2 Correspondence: Nutritional Genomics Center, Children’s Hospital of Oakland Research Institute, Oakland, CA 94609, USA. E-mail: bames@chori.org <h3>SPECIFIC AIMS</h3> Exposure to environmental stresses such as radiation, poor nutrition, or smoking can cause hazardous lesions in DNA, including double-strand breaks. The micronucleus test is an established method to analyze in vivo chromosomal damage. Conventional micronucleus assays performed in peripheral blood lymphocytes apply a chemical to block cytokinesis after a single cell division, and micronuclei (MN) are manually counted and scored using microscopy. In the current study, we sought to develop a simple assay to detect small increases in MN frequencies in people at risk. To accomplish this, rapid scoring of large numbers of cells was required. Micronuclei are particularly
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A simple assay for frequency of chromosome breaks and loss (micronuclei) by flow cytometry of human reticulocytes
Offer, Tal; Ho, Emily; Traber, Maret G.; Bruno, Richard S.; Kuypers, Frans A.; Ames, Bruce N.
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, Volume 19 (3): 485
Fed of American Socs for Experimental Biology – Mar 1, 2005
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