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Polymorphism of glutathione S-transferases as genetic risk
factors for the development of complications in type 2
Aysheh M. Hossaini, Israa M. Zamrroni, Reem A. Kashem, Zoiya F.M. Khan
Second Year Medical Students. Dubai Medical College for Girls. Dubai, UAE
Background: Diabetes is characterized by chronic hyperglycemia that produces dysregulation of cellular
metabolism and result in excess free radical production and oxidative stress. Glutathione S-transferases
(GSTs) are a family of multifunctional enzymes that work as antioxidants. Therefore, diminished
expression of GSTs may result in a reduced body defense against oxidative stress, followed by
development of diabetic complications.
Objectives: To investigate the possible association between GSTM1 and GSTT1 polymorphism and the
occurrence of complications in type 2 DM and to study the other risk factors for complications in DM.
Methods: Twenty noncomplicated type 2 diabetic patients and 40 complicated type 2 diabetic patients
were enrolled in the study. The GSTM1 and GSTT1 genotypes were identified by polymerase chain
reaction of peripheral blood DNA samples. Analysis of data was done by using SPSS (SPSS, Chicago, Ill).
Results: The frequencies of null GSTM1 and GSTT1 genotypes were 55% (11/20) and 40% (8/20),
respectively, in noncomplicated DM group. The frequencies of null GSTM1 and GSTT1 genotype in
complicated DM group were 57.5% (23/40) and 60% (24/40), respectively. The null GSTT1 genotype was
more prevalent in the group of complicated DM with odds ratio (odds ratio, 2.3; 95% confidence interval,
0.75-6.7) when compared with noncomplicated DM.
Conclusion: Our data provide evidence that diabetics with null GSTT1 genotypes are substantially at
higher risk for developing complications.
© 2008 Published by Elsevier Inc.
Source of research support: Dubai Medical College for Girls.
444 K. Shukri et al.