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Establishment of a recombination system Molecular genetics of the filamentous fungus A.nidulans has been facilitated by the availability of chromosome-specific cosmid libraries and their corresponding physical ...
and White, 1996). The majority of fungal AVR genes cloned to date are derived from fungi that colonize intercellular spaces in plant tissues. These genes were cloned by using reverse genetic approaches ...
., Gow, N. A., and Shaw, D. J., A Candida albicans genome project: cosmid contigs, physical mapping , and gene isolation. Fungal Genet. Biol., 21, 308–314 (1997). 7) Lu, S.-F., Tolstorukov, I. I., Anamnart ...
that the plasmid was present as a single copy contained within a 15-kb ClaI fragment (Figure 3 Figure 3. Open in new tabDownload slide Physical Maps of Plasmid -Tagged Mutant and Wild-Type Loci. The pAN7-1–tagged ...
) complemented the mutations in LTU61–LTU64 as expected, and also those in LTU65-LTU88. Thus, pVS1513 mimics the behavior of the cosmid recombinant, pVS1506. A physical map of the 8.8 kb fragment was constructed ...
to clone yeast DNA. The size of cosmid inserts was appropriate both for sequencing (vector DNA is short relative to inserts) and for constructing the high-resolution physical maps required to optimize ...
, many of which have antimicrobial properties. Similar to filamentous fungi , streptomycetes display two types of cell division [2]. During vegetative growth, infrequent cell division results ...
. 2010); however, SNP maps , other large-scale physical or genetic mapping data, or characterized strains with different genetic background are not available for S. macrospora. In this study, we describe ...
genes may belong to a class of proteins specific for filamentous fungi and function as novel virulence factors in fungal pathogens. INTRODUCTION The ascomycete Magnaporthe grisea is pathogenic ...
Plant pathogenic fungi must recognize susceptible hosts, overcome physical and chemical barriers, and colonize plant organs to exploit available nutrients. To survive, most fungi have evolved specialized ...
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