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A simple and inexpensive aqueous two ‐ phase affinity partitioning system using metal ligands was introduced to improve the selectivity of commercial papain extraction. Polyethylene glycol 4000 ...
Here we present data to demonstrate how partitioning of a hydrophilic enzyme can be directed to the hydrophobic detergent‐enriched phase of an aqueous two ‐ phase system by addition of short stretches ...
Abstract A halotolerant isolate Bacillus sp. L1 producing extracellular cellulase was isolated from Yuncheng, China. Production of the enzyme started from mid-exponential phase of bacterial growth ...
and extract from Kadsura Coccinea using hydrophobic organic- aqueous two - phase solvent systems J Liq Chromatogr Relat Technol 2009 32 2361 2371 10.1080/10826070903187981 Google Scholar Crossref Search ADS PubMed ...
information concerning haloarchaea as cellulase producers [27]. Since salt reduces water activity, a feature in common with organic solvent systems , enzymes from haloarchaea may possess organic solvent ...
reesei endoglucanase I (Cel7B) for enhanced partitioning in aqueous two - phase systems containing thermoseparating ethylene oxide–propylene oxide copolymers J Biotechnol 2001 87 179 191 10.1016/S0168-1656 ...
were concentrated, dissolved in water, and used for bioassays. Fractionation by solid- phase extraction using OASIS cartridges: The neutral aqueous layer (5 mL) obtained by the partitioning of the aqueous ...
WorldCat 45 Dogan N. , Tari C. ( 2008 ) Characterization of three- phase partitioned exo-polygalacturonase from Aspergillus sojae with unique properties . Biochem. Eng. J . 39 , 43 – 50 Google Scholar ...
of plant biotechnology applications. Hydrophobins are small and surface-active proteins derived from filamentous fungi that can be easily purified by a surfactant-based aqueous two - phase system ...
in aqueous two - phase systems J Chromatogr B 2007 854 273 278 10.1016/j.jchromb.2007.04.049 Google Scholar Crossref Search ADS WorldCat 27. Nakamura K , Fujii T, Kato Y, Asano Y, Cooper AJ Quantitation ofL ...
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