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of ribosome and subunits Observed precursors References RsgA E . coli Null mutation Decreased 70S ribosome relative to the subunits Pre-30S particle containing 17S RNA, which lacks some of r- proteins (5 ...
toxic protein ; the broadly conserved uncharacterized protein YggL is revealed to be a 50S subunit factor in assembled 70S ribosomes. The E . coli Grad-seq data are viewable in an online browser (https ...
peak D. Figure 10 View largeDownload slide Localization of the α-sarcin loop of 23S rRNA within the 50S subunit . The placement of labeled proteins L1, L2 , L5, L9, L15, L17 and L27 follows the model ...
that are responsible for protein synthesis in all living cells. Taking the bacterial ribosome as an example, it consists of a large subunit ( 50S ) and a small subunit (30S), which together form an intact 70S ribosome [1 ...
indicates a plasmid expressing chloramphenicol acetyltransferase (negative control protein ). Figure 1. Open in new tabDownload slide AL2 and L2 Abolish Complementation of E . coli HO4 by ADK ...
of the E . coli K12 proteome (4408 protein sequences), retrieved from the HAMAP database45 (based on UniProt46 release 2012_10). The UniRef 50 database (release 2012_10) used for profile–profile HMM ...
in other bacteria (like Staphylococcus epidermidis, Staphylococcus xylosus and Escherichia coli ), it is secreted to the culture medium only by S . aureus. Our results show that the ribosomal protein L2 has ...
TS40L ( 70 -kD), L (52-kD), and S (14.5-kD) subunits and Rubisco activase (42 kD) contents in the soluble (sol) and insoluble (pel) protein fractions of the leaves indicated in A. m, Marker proteins ; *, 54 ...
levels in ribosomal subunits . Ribosomal subunit fractions (from 30S to 50S ) corresponding to the profiles shown in Figure 2C were collected and r- proteins L13 and L2 were analyzed by Western blot ...
, and facilitates the unwinding of downstream structure to reach the tisB RBS (Figure 1A) (14). The importance of S1 for standby is intriguing. In E . coli , this protein has ubiquitous roles in translation (15–18 ...
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