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A in (i, j) retinal pigment epithelial cells, (k-l) human villous mesenchymal cells, and (m) the clade E virus competed with clade G in Vero cells. All competitive fitness assays were conducted ...
competed equally well with the untagged WT virus over 6 passages, demonstrating the selective neutrality of the marker (Fig 1A). In our serial passage competition assay , the change in relative frequency ...
further enhance virus fitness without compromising essential components of CH505 Env biology. We used short-term in vivo mutational selection and competition to identify a minimally adapted SHIV.C.CH505 ...
background, we rebuilt in parallel a set of non-reporter recCA09 variants. Dose-response assays against 4’-FlU demonstrated a 2 to 25-fold increase in EC99 concentrations of 4’-FlU against the different virus ...
passage competitive fitness assay between the TF and the CC virus , and a difference in fitness could only be visualised by passaging the mixture of cell-free viruses three times [29]. Transmitted HIV-1 ...
polymerase activity in a minigenome assay [17] as well as the slight growth advantage we measured in the virus at 24 hours in cell culture. Despite the fitness advantage of the P653L single mutant ...
the ratios obtained in the experiments. (D). A competition growth assay was performed in triplicate as described in Methods with approximately equal input copies of R880F and R463F viral stocks. Relative ...
at indicated dose, PR8-NSCE1-Gluc caused 60% lethality (Fig 2E). These data clearly demonstrate that the in vivo fitness of reporter IAVs can be, at least partially, restored by rebalancing the levels ...
virus in vitro and failed to stimulate the Ly49H reporter cell line BWZ-HD12 (data not shown). Virus determination in vivo Viral titers in mice were determined by standard plaque assay [57]. Briefly ...
ferret challenge model, which demonstrated that the reduced fitness of A/BOL13 led to inter-strain competition and loss of efficacy in multivalent vaccine formulations [19]. To improve on the reduced VE ...
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