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., Zhou,M., Reha-Krantz,L.J., Ide,H., Morrical,S.W. and Wallace,S.S. ( 1999) In vitro selection of sequence contexts which enhance bypass of abasic sites and tetrahydrofuran by T4 DNA polymerase holoenzyme ...
by formaldehyde, may promote further DNA damage. Abasic sites cause problems in sequence analysis. DNA polymerases have generally low bypass efficiencies at abasic sites (36), preventing amplification of DNA ...
. The frequency and the spectrum of the induced mutations depended on the sequence contexts and the lagging/leading synthesis. The MFs observed in these experiments were 0.1–0.8%, which are comparable with the MFs ...
. Notably, this subtle sequence - context variability can have significant, albeit moderate, effects on cleavage rates, a feature that might influence repair efficiency and the molecular consequences (16,17 ...
dinucleotide photoproducts. One notable exception is the trans-syn dimer of TT which has been reported to be bypassed to a significant extent under non-SOS conditions in one sequence context ( 23 ...
, which was previously identified using degenerate PCR primers designed to E.coliumuC and dinB sequences (11); the second is a related gene identified as yqjH in Halobacterium species NRC-1 (6,12). In fact ...
of the luciferase reporter gene. We have positioned this unique lesion in different sequence contexts and we have tested the effect of two promoters with different transcriptional strength on the level ...
in opposite base-, flanking sequence - and cell type-dependent manners (14,16–19). Large deletion mutations were induced by an abasic site and THF in primate and human cells, respectively (20,21). We found ...
used the bioinformatics results to guide our selection of two biologically relevant mtDNA sequences containing G4s for the in vitro biochemical experiments of mt G4 replication using purified recombinant ...
. Electrophoretic mobility shift assays and atomic force microscopy suggest transient complex formation between MUTYH and UV-DDB, which displaces MUTYH from abasic sites. Using single molecule fluorescence analysis ...
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