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displacement controlled device to uniaxially strain cell constructs under both static and dynamic compression and used this device to investigate gene expression in cell constructs. The device has a wide stroke ...
understanding of chondrocyte development and guide cartilage tissue engineering. Stem Cells 2015;33:3266–3280 miRNA target site enrichment analysis by Sylamer performed on the sorted gene list of expression ...
chondrocyte /MSC ratio of 63:1 had a gene expression profile similar to that of the chondrocyte control group, with a relatively reduced hypertrophic phenotype. Changes in Expression of CD166 and CD44 ...
development. (A): 2D Expansion of human chondrocytes in higher osmolarity medium increased aggrecan gene expression normalized to glyceraldehyde 3-phosphate dehydrogenase expression that was paralleled during ...
cells aggregates illustrate GAG (blue) and collagen (red) distribution after 21 days in 3D culture. Scale bar = 500 μm. Gene expression profiling of well-known chondrogenic marker genes revealed trends ...
the thermosensitive profile and printability Mouser et al. (2016)96 GelMA with gellan gum Extrusion bioprinting Equine articular chondrocytes Compressive modulus: 0.027–0.186 MPa Not reported Identified held stress ...
expression analysis of RFLCs. Total RNA was extracted during monolayer expansion over 10 passages under normoxia and hypoxia. The gene expression profile of rodent neonatal articular chondrocytes was used ...
(qRT-PCR) gene expression analysis for pluripotency gene Nanog, chondrogenic markers Sox9, Acan, and Col2a1, and Trpv4. Controls: undifferentiated iPSCs and isolated primary murine articular chondrocytes ...
of this IGF1 expression , and chondrocytes (expectedly) have high expression of IGF1R in the 3–7 days following compressive ACL rupture [46]. In the current porcine study, expression of IGF1 was elevated at 7 ...
pathologically affected. Remarkably, a side-by-side comparison with other cell types demonstrated that chondrocytes have substantially reduced capacity to endocytose rhARSB, together with low expression ...
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