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of cell death using a compatible three-dimensional tissue culture system ( multicellular spheroids) and compare the results obtained with those using two-dimensional monolayer cell culture . To do so, we ...
expressed as the secreted factor per 105 cells at the time of harvest. Western Blot The protein expression was determined at day 7 of cell culture . Monolayer and spheroid-derived ASCs were resuspended in cell ...
of this form of testing is such that it warrants further investment of both time and money to enhance the methodology such as to eliminate some of the levels of variability. angiogenesis , cancer, co- culture ...
deformations of an elastic substrate [24–26]. Initially applied for single cells, this method has been extended to monolayer cultures yielding a spatio-temporal map of traction stresses across a multicellular ...
and hASC-spheroids prepared by 3D culture in the HA gel. Spheroid formation promoted gene expression of some angiogenesis -related growth factors (VEGFA, VEGFB, HGF, PDGFA, PDGFC), anti-inflammatory factors ...
sprouts and tubes formed by vasculogenesis in such a homotypic co- culture system. In this study, ECs originally in the monolayer via sprouting angiogenesis , extended continuously into the bulk collagen ...
to be required for EC filopodial tip directionality during angiogenesis .36 VEGF also promotes von Willebrand factor release, integrin expression , interstitial collagenase expression , plasminogen activator (PA ...
relative to free doxorubicin. Results are means ± SD (n = 3). **P < 0.01, vs control group. Cytotoxicity evaluation on 3D multicellular tumour spheroids Monolayer or suspension cell cultures lack 3D ...
and monolayer sprouting assays, we evaluated differential gene expression for VEGFA, bFGF, and HIF-1α in the different groups and culture conditions using qPCR. After 48 hours, fibrin gels containing spheroids ...
endothelial growth factor of type A (VEGF-A) secretion profiles from osteosarcoma cells (MG-63) in monolayer and spheroid cultures are characterized. The MG-63 cell line is chosen as the in vitro model due ...
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