“Woah! It's like Spotify but for academic articles.”

Instant Access to Thousands of Journals for just $40/month

Get 2 Weeks Free

Evidence for participation of rage (receptor for advanced glycation end products), soluble rage (sRAGE) and S100A12 proteins, in control of the inflammatory response of amniotic cavity during pregnancy

Evidence for participation of rage (receptor for advanced glycation end products), soluble rage (sRAGE) and S100A12 proteins, in control of the inflammatory response of amniotic cavity during pregnancy <h5>Objective</h5> RAGE (NF-B activator) is a novel membrane cell-surface receptor with role in inflammation. S100A12 (proteomic biomarker of inflammation) is a ligand for RAGE. sRAGE competitively inhibits RAGE ligands. Studies were conducted to understand the relationships among RAGE, sRAGE and S100A12 in the amniotic fluid (AF) and fetal tissues of women with PTL in the presence or absence of intra-AF infection/inflammation (IAI/I).</P><h5>Study design</h5> AF was retrieved by amniocentesis in 93 consecutive women stratified as follows: i) +AFC: positive AF culture, n=20, GA=27.1±0.7wks; ii) −AFC: negative AF culture, n=30, GA=28.7±0.9 wks; iii) 2nd trim. control, n=15, GA=18.5±0.4wks; iv) 3rd trim. control, n=28, GA=36.7±0.4wks. sRAGE and IL-6 levels were measured by highly specific immunoassay. Each sample was subjected to mass spectrometry (SELDI) for detection of S100A12. Fetal membranes, cord and placenta were stained for RAGE and S100A12 protein.</P><h5>Results</h5> The S100A12 [10.4kDa] biomarker was present in 70%(14/20) of women with +AFC but only in 10%(3/30) of women with –AFC(p<0.001). Its presence correlated with AF levels of IL-6 (r=0.63, p<0.001) and AF WBC count (r=0.71, p<0.001). No control had the S100A12 SELDI biomarker peak. Levels of the inhibitor sRAGE were not influenced by presence or absence of IAI/I but increased directly with increasing GA (r=0.67, p<0.001) [Figure]. Staining for RAGE was present in all fetal membranes, and did not vary with IAI/I. Staining for S100A12 was selectively and markedly increased in the amnion and chorio-decidua of patients with IAI/I. </P><h5>Conclusion</h5> The proinflammatory ligand S100A12 is upregulated in the presence of IAI/I. Its receptor RAGE and inhibitor sRAGE are selectively present in fetal membranes and AF, respectively. The GA dependence of sRAGE may explain the higher incidence of IAI/I-related prematurity at earlier GAs.</P> http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Obstetrics and Gynecology Elsevier

Loading next page...
1 Page
 
/lp/elsevier/evidence-for-participation-of-rage-receptor-for-advanced-glycation-end-5zreg8zdwp

You're reading a free preview. Subscribe to read the entire article.

And millions more from thousands of peer-reviewed journals, for just $40/month

Get 2 Weeks Free

To be the best researcher, you need access to the best research

  • With DeepDyve, you can stop worrying about how much articles cost, or if it's too much hassle to order — it's all at your fingertips. Your research is important and deserves the top content.
  • Read from thousands of the leading scholarly journals from Springer, Elsevier, Nature, IEEE, Wiley-Blackwell and more.
  • All the latest content is available, no embargo periods.

Stop missing out on the latest updates in your field

  • We’ll send you automatic email updates on the keywords and journals you tell us are most important to you.
  • There is a lot of content out there, so we help you sift through it and stay organized.