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) were expressed in yeast , which produced 3 mg/L day of hydrocortisone , around sixfold lower than the A. orchidis system. The A. orchidis system (CYP5311B2, CPR and CYPB5) was further engineered ...
-reductase (DHCR7) and dehydrocholesterol 24-reductase (DHCR24) from Danio rerio, the cholesterol pathway was constructed in engineered yeast . Similar DHCR7 and DHCR24 enzymes from Xenopus laevis, humans ...
synthase XpkA A. nidulans xylulose-5-phosphate phosphoketolase α-KIV α-ketoisovalarate α-KMV alpha-keto-beta-methylvalerate α-KV α-Ketovalerate INTRODUCTION The yeast Saccharomyces cerevisiae, a preferred ...
). In addition, heterologous expressions of P450-CPR combinations are critical for complex triterpenoid production in yeast (Fukushima et al.2013). Hydrocortisone , a major mammalian adrenal glucocorticoid ...
human receptor protein and response elements to activate transcription of a reporter gene (Zacharewski, 1997). Thus, it is important to realize that yeast -based systems cannot explicitly identify ...
with cheW chemotaxis gene, double tac promoter, trpA terminator, phage M13 ori, lacIq, Ampr [2] pCWh1A1 pCWori+ containing a N-terminally modified human CYP1A1 gene and the full-length human CPR gene, does ...
, metabolic engineering, synthetic biology INTRODUCTION The baker's yeast Saccharomyces cerevisiae has long been used for its capacity to produce both beverage and fusel ethanol (McGovern 2009; Lee, Jellison ...
regions, and the PCR product can be transformed into the yeast for insertion by double -crossover. The efficiency of recombination in yeast also allows the use of nested primers or the assembly of two ...
need to improve the evaluation and classification of BRCA1 VUSs. To achieve this, various functional assays have been assessed, in order to make an accurate evaluation of the risk posed by newly ...
only very small serum volumes (maximally 30 μl), making them applicable for pediatric purposes as well. In addition to human serum we also tested fetal calf serum for estrogenic and androgenic activities ...
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