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Several approaches are available to achieve multi - transgene - stacking in plants for the introduction of complex or multiple traits. We used co-transformation with multiple plasmids using particle ...
in Escherichia coli, suitable to clone the extremely large resistance stack (37 kb all together) into a multi - transgene cassette. The cassette was then transferred into the wheat cells via a new high-efficiency ...
into plant cells (for review, see Halpin et al., 2001; Daniell and Dhingra, 2002; Halpin and Boerjan, 2003). Some of the approaches used for the production of transgenic plants carrying multiple new traits ...
(SS1+NS1) had a significant yield increase in more locations than either of the single allele transgenics . Multi -location analysis showed that transgenic plants of the heterozygous allele stack had ...
and Cas9 cassettes within these plants initiated multiple experiments for future use (gene stacking and CRISPR/Cas9 targeting). The study mainly focuses on developing Rec and Cas9 positive transgenic plants ...
the stacking of TUs by GreenBraiding, we constructed a proof-of-concept plant transformation vector containing four TUs. We aimed to express four fluorescent-tagged fusion proteins from four different promoters ...
of primary transformants were harvested from mature fertile plants and were employed for generation advancement. PCR screening and Southern blot analyses The presence and transmission of the transgene ...
cotton is managed using an IPM approach . The aim of this study was to establish if the insect community in transgenic Bt cotton differs from that in unsprayed conventional cotton and, further, to compare ...
of the major challenges that are still to be addressed in plant biotechnology are the development of a technology to combine several transgenic traits in a single plant by stacking a number of genes in the same ...
Abstract Agricultural pest infestation is as old as domestication of food crops and contributes a major share to the cost of crop production. In a transgenic pest control approach , plant production ...
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