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this hypothesis, a convenient and simple myoblast culture model using frozen embryonic myoblasts was developed, which resolved a number of problems inherent to cell primary culture . Biological assays on cultured ...
in D3 cells , compared with cells cultured under control conditions (Fig. 1B). Levels of miR294 and miR206, as well as myogenic factors, suggested significant differences between B8 and D3 cells . Thus, we ...
experimental conditions (24-hour exposure to 95% O2/5% CO2) at passage (P) 1 and 5. At each passage, cells were allowed to attach to 100 cm2 culture dishes and grow in 21% O2 before being subjected ...
by hypoxia. Moreover, the angiogenesis assay showed that condition media extracted from hypoxia-treated MDSPCs support endothelial cells ’ survival, migration and tube formation in vitro. The cultured cells ...
concentration of growth factors is sufficient to propagate myogenic progenitors. Human embryonic stem (ES) cell colonies were cultured as EZ spheres in either low (10 ng/ml each) or high (100 ng/ml each ...
-induced cultures were switched to myogenic differentiation conditions . For secondary transplantations, 5,000 GFP+ sorted cells per 10 μl PBS were injected into left TAs of secondary mdx mice, whereas right ...
) culture conditions through examination of the cell proliferation and the expression of myogenic genes. The data showed that 1-day-old spheroids had more intense expression of MyoD gene with respect ...
; myostatin; and TGFβ1 mRNA expression during myogenesis in PEMC cultures . MATERIALS AND METHODS Porcine Embryonic Myogenic Cell Cultures All procedures involving animals were approved by the local ...
-renewable mouse muscle stem cells . In summary, these results offer insight into the heterogeneity of BSC and provide evidence for subtle differences between rodent and bovine myogenic precursors. INTRODUCTION ...
‐cadherin protein in myogenic cells during prenatal mouse development and differentiation of embryonic stem cells in culture . Dev Dyn 1994 ; 201 : 245 – 259 . Google Scholar OpenURL Placeholder Text WorldCat ...
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